deeptools

A set of command-line tools for analysing next-generation sequencing data, such as DNA or RNA reads. It converts alignment files into coverage tracks, checks data quality, compares samples, and makes plots.

In plain words
What is it for?
Use it to process ChIP-seq, RNA-seq, ATAC-seq, and similar experiments; check replicates and sequencing depth; compare treatment with control; and plot signal around genes, peaks, or other genomic regions.
Why use it?
It brings common sequencing checks and visualisations into one workflow, so you do not need to build each analysis from separate tools.

Skill for Claude CodeCodex

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

agentmods
npx agentmods add skills/magic3007/dotfiles/deeptools
Any agent
npx skills add magic3007/dotfiles --skill deeptools
Clone the repo
git clone --depth 1 https://github.com/magic3007/dotfiles

Made for: Claude Code, Codex.

Per session 51 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 4,239 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. Scan, not verified.
Origin 97% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5 $0.00051 $0.04239
Opus 5 $0.00026 $0.02119
Sonnet 5 $0.00010 $0.00848
Haiku 4.5 $0.00005 $0.00424

Measured 2d ago against content hash b8b4340a55e6, method: parsed. Prices are Anthropic first-party input rates as of 2026-08-30, from the pricing page.

Security

Grade A, and why

deeptools scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 2d ago.

The scan reads SKILL.md. This mod also ships 2 executable files (scripts/validate_files.py, scripts/workflow_generator.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

This is a copy

97% identical to deeptools — 4 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

claude/skills/scientific-agent-skills/skills/deeptools/SKILL.md · 531 lines

How it starts

The opening of the file, as written. The whole thing — 531 lines — stays where its author put it; the contents beside it link to each section on GitHub.

deepTools: NGS Data Analysis Toolkit

Overview

deepTools is a comprehensive suite of Python command-line tools designed for processing and analyzing high-throughput sequencing data. Use deepTools to perform quality control, normalize data, compare samples, and generate publication-quality visualizations for ChIP-seq, RNA-seq, ATAC-seq, MNase-seq, and other NGS experiments.

Core capabilities:

  • Convert BAM alignments to normalized coverage tracks (bigWig/bedGraph)
  • Quality control assessment (fingerprint, correlation, coverage)
  • Sample comparison and correlation analysis
  • Heatmap and profile plot generation around genomic features
  • Enrichment analysis and peak region visualization

When to Use This Skill

This skill should be used when:

  • File conversion: "Convert BAM to bigWig", "generate coverage tracks", "normalize ChIP-seq data"
  • Quality control: "check ChIP quality", "compare replicates", "assess sequencing depth", "QC analysis"
  • Visualization: "create heatmap around TSS", "plot ChIP signal", "visualize enrichment", "generate profile plot"
  • Sample comparison: "compare treatment vs control", "correlate samples", "PCA analysis"
  • Analysis workflows: "analyze ChIP-seq data", "RNA-seq coverage", "ATAC-seq analysis", "complete workflow"
  • Working with specific file types: BAM files, bigWig files, BED region files in genomics context

Quick Start

For users new to deepTools, start with file validation and common workflows:

1. Validate Input Files

Before running any analysis, validate BAM, bigWig, and BED files using the validation script:

python scripts/validate_files.py --bam sample1.bam sample2.bam --bed regions.bed

This checks file existence, BAM indices, and format correctness.

2. Generate Workflow Template

For standard analyses, use the workflow generator to create customized scripts:

# List available workflows
python scripts/workflow_generator.py --list

# Generate ChIP-seq QC workflow
python scripts/workflow_generator.py chipseq_qc -o qc_workflow.sh \
    --input-bam Input.bam --chip-bams "ChIP1.bam ChIP2.bam" \
    --genome-size 2913022398

# Make executable and run
chmod +x qc_workflow.sh
./qc_workflow.sh

Read the full file on GitHub · 531 lines

Files

What ships with it

7 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 2d ago First seen · 531 lines · 51 tokens per session scan A b8b4340a55e6

Subscribe to this mod's changes

deeptools is a skill published in the GitHub repository magic3007/dotfiles (10 stars, last pushed 6d ago), licensed MIT. It adds 51 tokens to every session and 4,239 once invoked, about $0.0003 per session on Opus 5. A static security scan graded it A with 0 findings. It is 97% identical to deeptools, differing in 4 lines, and is treated as a copy.

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