Analyzes cfDNA fragment size distributions and fragmentomics features using FinaleToolkit or Griffin. Extracts nucleosome positioning patterns, fragment ratios, and DELFI-style fragmentation profiles for cancer detection. Use when leveraging fragment patterns for tumor detection or tissue-of-origin analysis.
Variant calling with GATK HaplotypeCaller following best practices. Covers germline SNP/indel calling, GVCF workflow for cohorts, joint genotyping, and variant quality score recalibration (VQSR). Use when calling variants with GATK HaplotypeCaller.
Skill "bio-genome-assembly-assembly-polishing" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-assembly-qc" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-contamination-detection" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-hifi-assembly" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-long-read-assembly" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-metagenome-assembly" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-scaffolding" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-assembly-short-read-assembly" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Design guides for cytosine and adenine base editing using editing window optimization and BE-Hive outcome prediction. Select optimal positions for C-to-T or A-to-G conversions without double-strand breaks. Use when designing base editor experiments for precise nucleotide changes.
Design guide RNAs for CRISPR-Cas9/Cas12a experiments using CRISPRscan and local scoring algorithms. Score guides for on-target activity using Rule Set 2 and Azimuth models. Use when designing sgRNAs for gene knockout, activation, or repression experiments.
Design homology-directed repair donor templates for CRISPR knock-ins using primer3-py. Create ssODN, dsDNA, or plasmid templates with optimized homology arms. Use when designing donor templates for precise insertions, tagging, or allele replacement.
Predict CRISPR off-target sites using Cas-OFFinder and CFD scoring algorithms. Identify potential unintended cleavage sites genome-wide and assess guide specificity. Use when evaluating guide RNA specificity or selecting guides with minimal off-target risk.
Design pegRNAs for prime editing using PrimeDesign algorithms. Generate spacer, PBS, and RT template sequences for precise genomic modifications without double-strand breaks. Use when designing prime editing experiments for precise insertions, deletions, or point mutations.
Skill "bio-genome-intervals-bed-file-basics" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-intervals-bigwig-tracks" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-intervals-coverage-analysis" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-intervals-gtf-gff-handling" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-intervals-interval-arithmetic" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-genome-intervals-proximity-operations" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Skill "bio-geo-data" from FreedomIntelligence/OpenClaw-Medical-Skills, covering copyright notice, copyright (c) 2026 md babu mia, phd, all rights reserved, this code is proprietary and confidential and provenance: authenticated by md babu mia.
Detect A/B compartments from Hi-C data using cooltools and eigenvector decomposition. Identify active (A) and inactive (B) chromatin compartments from contact matrices. Use when identifying A/B compartments from Hi-C data.
Process Hi-C read pairs using pairtools. Parse alignments, filter duplicates, classify pairs, and generate contact statistics from Hi-C sequencing data. Use when processing raw Hi-C read pairs.
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