tooluniverse-single-cell

tooluniverse-single-cell is a skill for Claude Code, Codex from AndyZhuang/Opentest. It costs 230 tokens per session (6,416 once invoked), scanned A, original, MIT.

A workflow for analyzing single-cell RNA sequencing data and other tables of gene-expression measurements. It can group similar cells, identify cell types, find genes that differ between groups, and study relationships between genes.

In plain words
What is it for?
Use it with h5ad files, 10X data, or CSV count matrices for quality checks, normalization, PCA, UMAP, t-SNE, clustering, cell-type annotation, differential expression, correlation, and batch correction.
Why use it?
It organizes the many steps needed to turn raw or processed expression data into interpretable cell populations and comparisons. It also supports common corrections and statistical tests when samples or conditions need to be compared.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it with h5ad files, 10X data, or CSV count matrices for quality checks, normalization, PCA, UMAP, t-SNE, clustering, cell-type annotation, differential expression, correlation, and batch correction.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/andyzhuang/opentest/tooluniverse-single-cell
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add AndyZhuang/Opentest --skill tooluniverse-single-cell
Clone the repo
git clone --depth 1 https://github.com/AndyZhuang/Opentest

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for tooluniverse-single-cell

README.md
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Your own site
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Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for tooluniverse-single-cell

Your own site · 80×15
<a href="https://agentmods.dev/skills/andyzhuang/opentest/tooluniverse-single-cell"><img src="https://agentmods.dev/badge/skills/andyzhuang/opentest/tooluniverse-single-cell.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 230 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 6,416 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00230 $0.06416
Opus 5 $0.00115 $0.03208
Sonnet 5 $0.00046 $0.01283
Haiku 4.5 $0.00023 $0.00642

Measured 8d ago against content hash 19a14ac6d317, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-11, from the pricing page.

Security

Grade A, and why

tooluniverse-single-cell scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 8d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

skills/labclaw/bio/tooluniverse-single-cell/SKILL.md · 720 lines

How it starts

The opening of the file, as written. The whole thing — 720 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Single-Cell Genomics and Expression Matrix Analysis

Comprehensive single-cell RNA-seq analysis and expression matrix processing using scanpy, anndata, scipy, and ToolUniverse. Designed for both full scRNA-seq workflows (raw counts to annotated cell types) and targeted expression-level analyses (per-cell-type DE, correlation, ANOVA, clustering).

IMPORTANT: This skill handles complex multi-workflow analysis. Most implementation details have been moved to references/ for progressive disclosure. This document focuses on high-level decision-making and workflow orchestration.


When to Use This Skill

Apply when users:

  • Have scRNA-seq data (h5ad, 10X, CSV count matrices) and want analysis
  • Ask about cell type identification, clustering, or annotation
  • Need differential expression analysis by cell type or condition
  • Want gene-expression correlation analysis (e.g., gene length vs expression by cell type)
  • Ask about PCA, UMAP, t-SNE for expression data
  • Need Leiden/Louvain clustering on expression matrices
  • Want statistical comparisons between cell types (t-test, ANOVA, fold change)
  • Ask about marker genes for cell populations
  • Need batch correction (Harmony, combat)
  • Want trajectory or pseudotime analysis
  • Ask about cell-cell communication (ligand-receptor interactions)
  • Questions mention "single-cell", "scRNA-seq", "cell type", "h5ad"
  • Questions involve immune cell types (CD4, CD8, CD14, CD19, monocytes, etc.)

BixBench Coverage: 18+ questions across 5 projects (bix-22, bix-27, bix-31, bix-33, bix-36)

NOT for (use other skills instead):

  • Bulk RNA-seq DESeq2 analysis only → Use tooluniverse-rnaseq-deseq2
  • Gene enrichment only (no expression data) → Use tooluniverse-gene-enrichment
  • VCF/variant analysis → Use tooluniverse-variant-analysis
  • Statistical modeling (regression, survival) → Use tooluniverse-statistical-modeling

Core Principles

  1. Data-first approach - Load, inspect, and validate data before any analysis
  2. AnnData-centric - All data flows through anndata objects for consistency
  3. Cell type awareness - Many questions require per-cell-type subsetting and analysis
  4. Statistical rigor - Proper normalization, multiple testing correction, effect sizes
  5. Scanpy standard pipeline - Follow established best practices for scRNA-seq
  6. Flexible input - Handle h5ad, 10X, CSV/TSV, pre-processed and raw data
  7. Question-driven - Parse what the user is actually asking and extract the specific answer
  8. Enrichment integration - Chain DE results into GO/KEGG/Reactome enrichment when requested
  9. Large dataset support - Efficient handling of datasets with >100k cells

Read the full file on GitHub · 720 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 8d ago First seen · 720 lines · 230 tokens per session scan A 19a14ac6d317

Subscribe to this mod's changes

tooluniverse-single-cell is a skill published in the GitHub repository AndyZhuang/Opentest (22 stars, last pushed 6mo ago), licensed MIT. It adds 230 tokens to every session and 6,416 once invoked, about $0.0011 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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