doubletfinder-remove-doublets

doubletfinder-remove-doublets is a skill for Claude Code from GGbond-bo/MemOmics-Agent. It costs 29 tokens per session (2,452 once invoked), scanned A, original, MIT.

A single-cell RNA sequencing workflow for detecting and removing doublets, which are droplets containing two cells instead of one. It uses Seurat and DoubletFinder to identify suspicious cells and produce filtered data and diagnostic plots.

In plain words
What is it for?
Use it to optimize detection settings, label likely doublets on UMAP plots, filter them, and save the cleaned object.
Why use it?
It helps prevent mixed-cell droplets from being mistaken for real cell types or states.

Skill for Claude Code

Written for Claude Code: when-to-use in frontmatter.

Good fit Use it to optimize detection settings, label likely doublets on UMAP plots, filter them, and save the cleaned object.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add GGbond-bo/MemOmics-Agent --skill doubletfinder-remove-doublets
Clone the repo
git clone --depth 1 https://github.com/GGbond-bo/MemOmics-Agent

Made for: Claude Code.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for doubletfinder-remove-doublets

README.md
[![agentmods](https://agentmods.dev/badge/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets/github.svg)](https://agentmods.dev/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets)
Your own site
<a href="https://agentmods.dev/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets"><img src="https://agentmods.dev/badge/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for doubletfinder-remove-doublets

Your own site · 80×15
<a href="https://agentmods.dev/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets"><img src="https://agentmods.dev/badge/skills/ggbond-bo/memomics-agent/doubletfinder-remove-doublets.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 29 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,452 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00029 $0.02452
Opus 5 $0.00015 $0.01226
Sonnet 5 $0.00006 $0.00490
Haiku 4.5 $0.00003 $0.00245

Measured 9d ago against content hash e6df2d20e17e, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

doubletfinder-remove-doublets scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (scripts/run.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

hermes_home/skills/bioinformatics/doubletfinder-remove-doublets/SKILL.md · 209 lines

How it starts

The opening of the file, as written. The whole thing — 209 lines — stays where its author put it; the contents beside it link to each section on GitHub.

DoubletFinder 去双胞

DoubletFinder通过模拟人工双胞在PC空间中检测doublets。参数自适应: pK通过BCparamOpt优化; 衰老样本→预期双胞数+20%; 免疫细胞丰富→+10%。所有10x数据都应做双胞检测

When to Use

scRNA-seq数据需去除双细胞

Triggers

  • DoubletFinder
  • 去双胞
  • doublet
  • 双细胞

Pipeline

  1. Seurat PCA预处理
  2. BCparamOpt优化pK
  3. 计算nExp_poi预期双胞数
  4. doubletFinder检测
  5. UMAP标注双胞
  6. subset过滤双胞
  7. 保存到doubletfinder/目录

Parameters

Parameter Default Notes
pN 0.25
pK auto(BCparamOpt)
nExp_poi auto(细胞数0.008千细胞数)

Parameter Adaptation: Adjust parameters based on tissue quality, species, and condition. Literature values take priority, then official defaults, then tissue-specific adjustments.

Dependencies

  • DoubletFinder
  • Seurat
  • spatstat

Outputs

  • 双胞标注对象
  • pK优化曲线
  • UMAP双胞图
  • 过滤后对象

Proven Scripts

Scripts that have been successfully executed and passed analysis review. These are automatically saved after successful runs.

Species Tissue Condition Date Score
(none yet)

Common Issues

Error Cause Solution
(accumulated from runs)

References

  • Source: Biomni-style (custom)
  • Category: decontamination
  • Language: R

🗣️ 辩论机制(debate_analysis)

本 skill 在执行后,如果涉及参数选择、方法决策、结果判断等不确定环节,必须调用 工具进行多角色辩论。

辩论规则

  • 正方 3 位专业编辑(各自独立,互相看不到):生物学编辑 / 统计学编辑 / 生信编辑
  • 反方 4 位专业编辑(各自独立,互相看不到,也看不到正方):生物学编辑 / 统计学编辑 / 生信编辑 / 历史经验编辑
  • 裁判:看到所有 7 方论点后给出裁决 + 置信度(高/中/低)
  • 上下文隔离:每个编辑是独立的 LLM API 调用,messages 只包含自己的 prompt

触发场景

  • 参数选择有多个合理选项时(如分辨率 0.4 vs 0.6 vs 0.8)
  • 结果可能受方法选择影响时(如不同注释方法给出不同结果)
  • 生物结论需要验证可靠性时
  • QC 阈值不确定时(如 MT% 阈值 10% vs 15% vs 20%)

不触发场景

  • 参数有明确知识库推荐且无争议时
  • 纯计算步骤(如保存文件、读取数据)

🔒 审查机制(rail_review)

本 skill 执行代码前必须调用 进行前置审查,执行后必须调用 进行后置审查。

审查内容

  • pre 审查:环境检查(包是否安装)→ 参数校验(参数是否合理)→ 代码审查(语法/逻辑)→ 硬件检查(内存/GPU是否够)
  • post 审查:结果质量评估(输出是否合理)→ 图表检查(图是否生成)→ 数值检查(细胞数/基因数是否异常)→ 错误检查(有无 warning/error)

Read the full file on GitHub · 209 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 209 lines · 29 tokens per session scan A e6df2d20e17e

Subscribe to this mod's changes

doubletfinder-remove-doublets is a skill published in the GitHub repository GGbond-bo/MemOmics-Agent (19 stars, last pushed 2d ago), licensed MIT. It adds 29 tokens to every session and 2,452 once invoked, about $0.0001 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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