bio-phylo-modern-tree-inference

bio-phylo-modern-tree-inference is a skill for Claude Code, Codex from thesecondfox/skill. It costs 52 tokens per session (2,259 once invoked), scanned A, original, MIT.

A guide to building maximum-likelihood phylogenetic trees from aligned biological sequences. A phylogenetic tree is a diagram that estimates how organisms or sequences are related.

In plain words
What is it for?
Use it with IQ-TREE2 or RAxML-ng for model selection, tree inference, bootstrap support, and partitioned analyses.
Why use it?
It helps compare possible evolutionary histories and measure how strongly the data support each branch.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it with IQ-TREE2 or RAxML-ng for model selection, tree inference, bootstrap support, and partitioned analyses.

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Install with agentmods
npx agentmods add skills/thesecondfox/skill/bio-phylogenetics-modern-tree-inference
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add thesecondfox/skill --skill bio-phylogenetics-modern-tree-inference
Clone the repo
git clone --depth 1 https://github.com/thesecondfox/skill

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-phylo-modern-tree-inference

README.md
[![agentmods](https://agentmods.dev/badge/skills/thesecondfox/skill/bio-phylogenetics-modern-tree-inference.svg)](https://agentmods.dev/skills/thesecondfox/skill/bio-phylogenetics-modern-tree-inference)
Your own site
<a href="https://agentmods.dev/skills/thesecondfox/skill/bio-phylogenetics-modern-tree-inference"><img src="https://agentmods.dev/badge/skills/thesecondfox/skill/bio-phylogenetics-modern-tree-inference.svg" alt="Measured on agentmods" height="20"></a>
Per session 52 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,259 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00052 $0.02259
Opus 5 $0.00026 $0.01130
Sonnet 5 $0.00010 $0.00452
Haiku 4.5 $0.00005 $0.00226

Measured 4d ago against content hash d2b8746ee522, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-08, from the pricing page.

Security

Grade A, and why

bio-phylo-modern-tree-inference scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 4d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Common_Skills/bio-phylogenetics-modern-tree-inference/SKILL.md · 269 lines

How it starts

The opening of the file, as written. The whole thing — 269 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: IQ-TREE 2.2+

Before using code patterns, verify installed versions match. If versions differ:

  • CLI: <tool> --version then <tool> --help to confirm flags

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Modern ML Tree Inference

"Infer a maximum likelihood tree from my alignment" → Build publication-quality ML trees with automatic substitution model selection and ultrafast bootstrap support assessment.

  • CLI: iqtree2 -s alignment.fa -m MFP -B 1000 (IQ-TREE2)
  • CLI: raxml-ng --all --msa alignment.fa --model GTR+G --bs-trees 100 (RAxML-ng)

Build maximum likelihood phylogenetic trees with automatic model selection and ultrafast bootstrap.

IQ-TREE2 Basic Usage

# Simple ML tree with automatic model selection
iqtree2 -s alignment.fasta -m MFP -B 1000 -T AUTO

# -s: input alignment
# -m MFP: ModelFinder Plus (automatic model selection + tree inference)
# -B 1000: 1000 ultrafast bootstrap replicates (minimum recommended for publication)
# -T AUTO: automatic thread detection

IQ-TREE2 Output Files

File Description
.treefile Best ML tree in Newick format
.iqtree Full analysis report with model parameters
.log Run log
.contree Consensus tree with bootstrap support
.splits.nex Bootstrap splits in Nexus format
.model.gz Model parameters
.bionj Initial BIONJ tree
.mldist ML distance matrix
.ckp.gz Checkpoint file for resuming

Model Selection

# ModelFinder only (no tree inference)
iqtree2 -s alignment.fasta -m MF

# Use specific model
iqtree2 -s alignment.fasta -m GTR+G4 -B 1000

# Test only specific models
iqtree2 -s alignment.fasta -m MF -mset GTR,HKY,K2P

# Protein models
iqtree2 -s protein.fasta -m MFP -B 1000 -st AA

Common DNA Substitution Models

Model Parameters Use Case
JC Equal rates Very simple, rarely appropriate
K2P/K80 Ti/Tv ratio Simple, some rate variation
HKY Ti/Tv + base freq Moderate complexity
GTR 6 rates + base freq Most general, recommended default
+G4 Gamma rate variation 4 discrete rate categories
+I Invariant sites Sites that never change
+R4 FreeRate model More flexible than Gamma

Read the full file on GitHub · 269 lines

Files

What ships with it

1 file beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 4d ago First seen · 269 lines · 52 tokens per session scan A d2b8746ee522

Subscribe to this mod's changes

bio-phylo-modern-tree-inference is a skill published in the GitHub repository thesecondfox/skill (3 stars, last pushed 5mo ago), licensed MIT. It adds 52 tokens to every session and 2,259 once invoked, about $0.0003 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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