nextflow-development

A workflow for running nf-core bioinformatics pipelines with Nextflow on sequencing data. It covers RNA-seq for gene activity, whole-genome or exome sequencing for DNA variants, and ATAC-seq for open chromatin, using local files or public GEO/SRA datasets.

In plain words
What is it for?
Running analyses such as gene-expression and differential-expression studies, variant calling, and chromatin-accessibility analysis from FASTQ files or public sequencing datasets.
Why use it?
It guides researchers through data acquisition, environment checks, pipeline selection, configuration, and output verification without requiring specialised bioinformatics training.

Skill for Claude CodeCodex

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

agentmods
npx agentmods add skills/anthropics/knowledge-work-plugins/nextflow-development
Any agent
npx skills add anthropics/knowledge-work-plugins --skill nextflow-development
Clone the repo
git clone --depth 1 https://github.com/anthropics/knowledge-work-plugins

Made for: Claude Code, Codex.

Per session 95 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,354 The whole file, excluding the scripts and references it only reads on demand.
Security scan D 3 findings. Scan, not verified.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5 $0.00095 $0.02354
Opus 5 $0.00048 $0.01177
Sonnet 5 $0.00019 $0.00471
Haiku 4.5 $0.00010 $0.00235

Measured 2d ago against content hash f30017df5d49, method: parsed. Prices are Anthropic first-party input rates as of 2026-08-30, from the pricing page.

Security

Grade D, and why

nextflow-development scanned grade D with 3 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 2d ago.

The scan reads SKILL.md. This mod also ships 10 executable files (scripts/check_environment.py, scripts/detect_data_type.py, scripts/generate_samplesheet.py, …), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Asks for rootmediumPrivilege escalation

A mod that escalates privileges can change anything on the machine, not only the project.

| Permission denied | `sudo usermod -aG docker $USER` then re-login |

Downloads and executes remote codehighSupply chain

curl | sh runs whatever the server returns today, which is not necessarily what it returned when this was reviewed.

| Not installed | `curl -s https://get.nextflow.io \| bash && mv nextflow ~/bin/` |

Makes network callslowCapability

Not a fault in itself. Listed so you know the mod talks to something, and to what.

| Not installed | `curl -s https://get.nextflow.io \| bash && mv nextflow ~/bin/` |
Origin

Copies of this mod

3 near-identical copies found in the catalogue:

bio-research/skills/nextflow-development/SKILL.md · 291 lines

How it starts

The opening of the file, as written. The whole thing — 291 lines — stays where its author put it; the contents beside it link to each section on GitHub.

nf-core Pipeline Deployment

Run nf-core bioinformatics pipelines on local or public sequencing data.

Target users: Bench scientists and researchers without specialized bioinformatics training who need to run large-scale omics analyses—differential expression, variant calling, or chromatin accessibility analysis.

Workflow Checklist

- [ ] Step 0: Acquire data (if from GEO/SRA)
- [ ] Step 1: Environment check (MUST pass)
- [ ] Step 2: Select pipeline (confirm with user)
- [ ] Step 3: Run test profile (MUST pass)
- [ ] Step 4: Create samplesheet
- [ ] Step 5: Configure & run (confirm genome with user)
- [ ] Step 6: Verify outputs

Step 0: Acquire Data (GEO/SRA Only)

Skip this step if user has local FASTQ files.

For public datasets, fetch from GEO/SRA first. See references/geo-sra-acquisition.md for the full workflow.

Quick start:

# 1. Get study info
python scripts/sra_geo_fetch.py info GSE110004

# 2. Download (interactive mode)
python scripts/sra_geo_fetch.py download GSE110004 -o ./fastq -i

# 3. Generate samplesheet
python scripts/sra_geo_fetch.py samplesheet GSE110004 --fastq-dir ./fastq -o samplesheet.csv

DECISION POINT: After fetching study info, confirm with user:

  • Which sample subset to download (if multiple data types)
  • Suggested genome and pipeline

Then continue to Step 1.


Step 1: Environment Check

Run first. Pipeline will fail without passing environment.

python scripts/check_environment.py

All critical checks must pass. If any fail, provide fix instructions:

Docker issues

Problem Fix
Not installed Install from https://docs.docker.com/get-docker/
Permission denied sudo usermod -aG docker $USER then re-login
Daemon not running sudo systemctl start docker

Nextflow issues

Problem Fix
Not installed curl -s https://get.nextflow.io | bash && mv nextflow ~/bin/
Version < 23.04 nextflow self-update

Read the full file on GitHub · 291 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 2d ago First seen · 291 lines · 95 tokens per session scan D f30017df5d49

Subscribe to this mod's changes

nextflow-development is a skill published in the GitHub repository anthropics/knowledge-work-plugins (23,791 stars, last pushed today), licensed Apache-2.0. It adds 95 tokens to every session and 2,354 once invoked, about $0.0005 per session on Opus 5. A static security scan graded it D with 3 findings (asks for root, downloads and executes remote code, makes network calls). No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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