Upstream Processing Skills Index

Upstream Processing Skills Index is a skill for Claude Code, Codex from aristoteleo/PantheonOS. It costs 40 tokens per session (510 once invoked), scanned A, original, BSD-2-Clause.

A collection of workflows for processing raw single-cell and spatial omics data before standard analysis. It covers sequencing files, barcode handling, read alignment, image registration, cell segmentation, and technology-specific pipelines.

In plain words
What is it for?
Processing Open-ST data, running nf-core bioinformatics pipelines, aligning reads and images, assigning transcripts to cells, registering spatial coordinates, and preparing data for downstream analysis.
Why use it?
Raw sequencing and imaging data are not ready for clustering or biological interpretation. These steps turn them into analysis-ready count tables with spatial locations when available.

Skill for Claude CodeCodex

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

agentmods
npx agentmods add skills/aristoteleo/pantheonos/upstream_processing
Any agent
npx skills add aristoteleo/PantheonOS --skill upstream_processing
Clone the repo
git clone --depth 1 https://github.com/aristoteleo/PantheonOS

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for Upstream Processing Skills Index

README.md
[![agentmods](https://agentmods.dev/badge/skills/aristoteleo/pantheonos/upstream_processing.svg)](https://agentmods.dev/skills/aristoteleo/pantheonos/upstream_processing)
Your own site
<a href="https://agentmods.dev/skills/aristoteleo/pantheonos/upstream_processing"><img src="https://agentmods.dev/badge/skills/aristoteleo/pantheonos/upstream_processing.svg" alt="Measured on agentmods" height="20"></a>
Per session 40 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 510 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. Scan, not verified.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5 $0.00040 $0.00510
Opus 5 $0.00020 $0.00255
Sonnet 5 $0.00008 $0.00102
Haiku 4.5 $0.00004 $0.00051

Measured 4d ago against content hash 544d73fbdf25, method: parsed. Prices are Anthropic first-party input rates as of 2026-08-30, from the pricing page.

Security

Grade A, and why

Upstream Processing Skills Index scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 4d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

pantheon/factory/templates/skills/omics/upstream_processing/SKILL.md · 59 lines

How it starts

The opening of the file, as written. The whole thing — 59 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Upstream Processing Skills

Skills and workflows for upstream data processing steps that precede standard single-cell analysis (QC, normalization, clustering, etc.). These cover technology-specific pipelines from raw sequencing data to analysis-ready count matrices with spatial coordinates.

Available Skills

OpenST

Open-ST is an open-source spatial transcriptomics technology that captures transcriptome-wide data at sub-cellular resolution. The computational pipeline covers flow cell barcode preprocessing, transcriptomic alignment via spacemake, image-to-coordinate registration, cell segmentation, and 3D reconstruction.

Skill directory: openst/

When to use:

  • Processing raw Open-ST data from BCL files to spatially-resolved h5ad
  • Aligning transcriptomic coordinates to H&E tissue images
  • Cell segmentation and transcript-to-cell assignment
  • 3D reconstruction from serial tissue sections

nf-core Pipelines

nf-core is a community-driven collection of 143+ curated Nextflow pipelines for bioinformatics. Skills cover installation, configuration, and pipeline-specific guides for transcriptomics, spatial omics, epigenomics, and variant calling.

Skill directory: nfcore/

When to use:

  • Processing scRNA-seq data (10x, Drop-seq, Smart-seq) with nf-core/scrnaseq
  • Processing spatial transcriptomics (Visium, Xenium, MERSCOPE) with nf-core pipelines
  • Processing bulk RNA-seq, ATAC-seq, ChIP-seq, CUT&Run, or methylation data
  • Variant calling from WGS/WES with nf-core/sarek
  • Setting up Nextflow and nf-core on HPC clusters or cloud environments

Using Skills

  1. Identify your technology: Find the relevant sub-directory for your spatial/sequencing platform
  2. Load skill files: Read the full skill documents for step-by-step guidance
  3. Follow the pipeline: Upstream processing is sequential; follow stages in order
  4. Proceed to downstream analysis: After generating the count matrix, use the main omics skills for QC, clustering, etc.

Read the full file on GitHub · 59 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 4d ago First seen · 59 lines · 40 tokens per session scan A 544d73fbdf25

Subscribe to this mod's changes

Upstream Processing Skills Index is a skill published in the GitHub repository aristoteleo/PantheonOS (482 stars, last pushed yesterday), licensed BSD-2-Clause. It adds 40 tokens to every session and 510 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

Related

Other skills, from other repositories