upstream_skills

upstream_skills is a skill for Claude Code, Codex from aristoteleo/PantheonOS. It costs 0 tokens per session (1,199 once invoked), scanned A, original, BSD-2-Clause.

An index of written workflows for processing biological sequencing data before downstream analysis. It covers methods such as RNA sequencing, ATAC sequencing, single-cell data, and spatial transcriptomics.

In plain words
What is it for?
Use it to guide quality checks, read processing, alignment, peak detection, gene-expression analysis, cell-type annotation, and spatial-data processing.
Why use it?
It helps an agent find the appropriate workflow for a type of experiment instead of starting with unsuitable or incomplete processing steps.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to guide quality checks, read processing, alignment, peak detection, gene-expression analysis, cell-type annotation, and spatial-data processing.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/aristoteleo/pantheonos/upstream_skills
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add aristoteleo/PantheonOS --skill upstream_skills
Clone the repo
git clone --depth 1 https://github.com/aristoteleo/PantheonOS

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for upstream_skills

README.md
[![agentmods](https://agentmods.dev/badge/skills/aristoteleo/pantheonos/upstream_skills/github.svg)](https://agentmods.dev/skills/aristoteleo/pantheonos/upstream_skills)
Your own site
<a href="https://agentmods.dev/skills/aristoteleo/pantheonos/upstream_skills"><img src="https://agentmods.dev/badge/skills/aristoteleo/pantheonos/upstream_skills/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for upstream_skills

Your own site · 80×15
<a href="https://agentmods.dev/skills/aristoteleo/pantheonos/upstream_skills"><img src="https://agentmods.dev/badge/skills/aristoteleo/pantheonos/upstream_skills.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 0 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,199 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00000 $0.01199
Opus 5 $0.00000 $0.00600
Sonnet 5 $0.00000 $0.00240
Haiku 4.5 $0.00000 $0.00120

Measured 13d ago against content hash f9d01bc6456b, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

upstream_skills scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 13d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

examples/fastq_processing/upstream_skills/SKILL.md · 158 lines

How it starts

The opening of the file, as written. The whole thing — 158 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Upstream Skills Index

This directory contains bioinformatics upstream analysis skills for agent-assisted data processing. Each skill provides workflow guidance, code templates, and best practices for specific analysis types.

Available Skills

Skill File Description Key Use Cases
ATAC-seq atac.md Bulk ATAC-seq analysis pipeline Chromatin accessibility profiling, peak calling, motif analysis
RNA-seq rna.md Bulk RNA-seq analysis pipeline Transcriptome profiling, gene expression quantification
scATAC-seq scatac.md Single-cell ATAC-seq analysis Single-cell chromatin accessibility, cellranger-atac processing
scRNA-seq scrna.md Single-cell RNA-seq analysis Single-cell transcriptomics, cell type annotation with LLM
Spatial spatial.md Spatial transcriptomics analysis Visium HD bin-to-cell conversion, spatial cell segmentation

Skill Summaries

ATAC-seq (atac.md)

Purpose: Complete bulk ATAC-seq data processing from raw FASTQ files to peak calls and downstream analysis.

Key Workflows:

  • Quality control with FastQC
  • Adapter trimming with Trim Galore
  • Genome alignment with Bowtie2/BWA
  • BAM filtering and duplicate removal
  • Peak calling with MACS2/Genrich
  • Coverage track generation
  • Motif analysis with HOMER

Tools Used: FastQC, Trim Galore, Bowtie2, BWA, samtools, Picard, MACS2, Genrich, deepTools, HOMER


RNA-seq (rna.md)

Purpose: Complete bulk RNA-seq data processing from raw FASTQ files to gene expression quantification.

Key Workflows:

  • Quality control with FastQC
  • Adapter trimming
  • Genome alignment with STAR/HISAT2
  • Expression quantification with featureCounts
  • BAM processing and QC
  • Coverage track generation

Tools Used: FastQC, Trim Galore, STAR, HISAT2, featureCounts, samtools, MultiQC


scATAC-seq (scatac.md)

Purpose: Single-cell ATAC-seq data processing using 10X Genomics cellranger-atac with downstream analysis.

Read the full file on GitHub · 158 lines

Files

What ships with it

5 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 13d ago First seen · 158 lines · 0 tokens per session scan A f9d01bc6456b

Subscribe to this mod's changes

upstream_skills is a skill published in the GitHub repository aristoteleo/PantheonOS (485 stars, last pushed today), licensed BSD-2-Clause. It costs nothing until one of its globs matches a file; then it loads 1,199 tokens. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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