bio-clip-seq-clip-peak-calling

bio-clip-seq-clip-peak-calling is a skill for Claude Code, Codex from GPTomics/bioSkills. It costs 129 tokens per session (7,450 once invoked), scanned A, original, MIT.

A guide to finding protein–RNA binding sites in CLIP-seq data, which records where RNA-binding proteins attach to RNA. It explains how to choose and use different peak callers on alignment files and control samples.

In plain words
What is it for?
Use it to analyze HITS-CLIP, iCLIP, eCLIP, or PAR-CLIP data, compare peak-calling tools, and apply control-based thresholds.
Why use it?
It helps you select a suitable analysis method for the type of CLIP experiment and avoid using command options that do not match installed software versions.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one. Also seen: positional $N argument.

Good fit Use it to analyze HITS-CLIP, iCLIP, eCLIP, or PAR-CLIP data, compare peak-calling tools, and apply control-based thresholds.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/gptomics/bioskills/clip-peak-calling
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add GPTomics/bioSkills --skill clip-peak-calling
Clone the repo
git clone --depth 1 https://github.com/GPTomics/bioSkills

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-clip-seq-clip-peak-calling

README.md
[![agentmods](https://agentmods.dev/badge/skills/gptomics/bioskills/clip-peak-calling/github.svg)](https://agentmods.dev/skills/gptomics/bioskills/clip-peak-calling)
Your own site
<a href="https://agentmods.dev/skills/gptomics/bioskills/clip-peak-calling"><img src="https://agentmods.dev/badge/skills/gptomics/bioskills/clip-peak-calling/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-clip-seq-clip-peak-calling

Your own site · 80×15
<a href="https://agentmods.dev/skills/gptomics/bioskills/clip-peak-calling"><img src="https://agentmods.dev/badge/skills/gptomics/bioskills/clip-peak-calling.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 129 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 7,450 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00129 $0.07450
Opus 5 $0.00064 $0.03725
Sonnet 5 $0.00026 $0.01490
Haiku 4.5 $0.00013 $0.00745

Measured 7d ago against content hash 4c0704d0cfd4, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-10, from the pricing page.

Security

Grade A, and why

bio-clip-seq-clip-peak-calling scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 7d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (examples/call_peaks.sh), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

Copies of this mod

1 near-identical copy found in the catalogue:

clip-seq/clip-peak-calling/SKILL.md · 359 lines

How it starts

The opening of the file, as written. The whole thing — 359 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: CLIPper 2.0+, PureCLIP 1.3.1+, Piranha 1.2.1+, omniCLIP 0.2.0+, CTK 1.1.4+, CLAM 1.2+, Paraclu 9+, Skipper (commit 2023.05+), MACS3 3.0+, bedtools 2.31+, samtools 1.19+, idr 2.0.4+.

Before using code patterns, verify installed versions match. If versions differ:

  • Python: pip show <package> then help(module.function) to check signatures
  • CLI: <tool> --version then <tool> --help to confirm flags

If code throws unexpected errors, introspect the installed binary and adapt the example to match the actual CLI rather than retrying. PureCLIP 2.x changed several flag names; Skipper is distributed as a Snakemake workflow with frequently-evolving paths.

CLIP-seq Peak Calling

"Call protein-RNA binding sites from my deduplicated CLIP BAM" -> Identify regions where read pile-up (and, for iCLIP/eCLIP, single-nucleotide truncations) exceed background from a size-matched input (SMInput) control. The choice of peak caller depends on (a) the CLIP variant (HITS-CLIP, iCLIP, eCLIP, PAR-CLIP), (b) whether SMInput is available, (c) the RBP binding mode (narrow motif vs broad zones vs repeat-binding), and (d) the goal (publication-comparable ENCODE peaks vs single-nucleotide crosslink sites vs high-recall site lists).

  • CLI (ENCODE eCLIP canonical): clipper -b dedup.bam -s hg38 -o peaks.bed --save-pickle --FDR-alpha 0.05 --superlocal then eclip-norm peaks.bed -i sminput.bam for log2 fold-change against SMInput (--FDR-alpha is the CLIPper flag for the FDR cutoff; older docs sometimes shorten to --FDR)
  • CLI (single-nucleotide crosslink sites, iCLIP/eCLIP): pureclip -i dedup.bam -bai dedup.bam.bai -g genome.fa -ibam sminput.bam -ibai sminput.bam.bai -o sites.bed -or regions.bed -nt 8 -dm 8
  • CLI (high-recall, beta-binomial windowed; needs SMInput): Skipper Snakemake workflow with config matching cell type and SMInput BAM
  • CLI (no SMInput, no truncation): Piranha -b 50 -p 0.01 -d ZeroTruncatedNegativeBinomial -s -o peaks.bed dedup.bam (Piranha takes the BAM as a positional argument, not via -s)
  • CLI (CIMS/CITS single-nt from CTK): tag2cluster.pl dedup.bed cluster.bed --multi-tag-method coverage; then bedExtractCIMS.pl cluster.bed cims.bed
  • CLI (multi-mapper rescue for repeat-binding RBPs): CLAM peakcaller -i unique.bam multimap.bam -o clam_out_dir/ --gtf gencode.gtf (CLAM peakcaller writes peaks into an OUTPUT DIRECTORY, not a single file)

Read the full file on GitHub · 359 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 7d ago First seen · 359 lines · 129 tokens per session scan A 4c0704d0cfd4

Subscribe to this mod's changes

bio-clip-seq-clip-peak-calling is a skill published in the GitHub repository GPTomics/bioSkills (1,199 stars, last pushed 26d ago), licensed MIT. It adds 129 tokens to every session and 7,450 once invoked, about $0.0006 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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