bio-clip-seq-crosslink-site-detection

bio-clip-seq-crosslink-site-detection is a skill for Claude Code, Codex from GPTomics/bioSkills. It costs 117 tokens per session (5,541 once invoked), scanned A, original, MIT.

A workflow for locating the exact RNA base where an RNA-binding protein crosslinked to its target. It uses read truncations or characteristic mutations found in different CLIP-seq protocols.

In plain words
What is it for?
Use it to detect crosslink sites in iCLIP, eCLIP, HITS-CLIP, or PAR-CLIP data and prepare them for motif, allele-specific, or variant-effect analysis.
Why use it?
Broad binding regions are often too imprecise for motif analysis or variant studies. Single-base sites provide a clearer position for downstream interpretation.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one. Also seen: positional $N argument.

Good fit Use it to detect crosslink sites in iCLIP, eCLIP, HITS-CLIP, or PAR-CLIP data and prepare them for motif, allele-specific, or variant-effect analysis.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/gptomics/bioskills/crosslink-site-detection
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add GPTomics/bioSkills --skill crosslink-site-detection
Clone the repo
git clone --depth 1 https://github.com/GPTomics/bioSkills

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-clip-seq-crosslink-site-detection

README.md
[![agentmods](https://agentmods.dev/badge/skills/gptomics/bioskills/crosslink-site-detection/github.svg)](https://agentmods.dev/skills/gptomics/bioskills/crosslink-site-detection)
Your own site
<a href="https://agentmods.dev/skills/gptomics/bioskills/crosslink-site-detection"><img src="https://agentmods.dev/badge/skills/gptomics/bioskills/crosslink-site-detection/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-clip-seq-crosslink-site-detection

Your own site · 80×15
<a href="https://agentmods.dev/skills/gptomics/bioskills/crosslink-site-detection"><img src="https://agentmods.dev/badge/skills/gptomics/bioskills/crosslink-site-detection.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 117 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 5,541 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00117 $0.05541
Opus 5 $0.00059 $0.02771
Sonnet 5 $0.00023 $0.01108
Haiku 4.5 $0.00012 $0.00554

Measured 7d ago against content hash e4ddc2272c92, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-10, from the pricing page.

Security

Grade A, and why

bio-clip-seq-crosslink-site-detection scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 7d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (examples/detect_crosslinks.sh), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

Copies of this mod

1 near-identical copy found in the catalogue:

clip-seq/crosslink-site-detection/SKILL.md · 260 lines

How it starts

The opening of the file, as written. The whole thing — 260 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: PureCLIP 1.3.1+, CTK 1.1.4+, PARalyzer 1.5+, wavClusteR 2.34+, pyCRAC 1.5+, samtools 1.19+, bedtools 2.31+, pysam 0.22+, R 4.3+.

Before using code patterns, verify installed versions match. If versions differ:

  • CLI: <tool> --version then <tool> --help to confirm flags
  • Python: pip show <package> then help(module.function) to check signatures
  • R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws unexpected errors, introspect the installed tool and adapt the example to match the actual CLI rather than retrying.

"Detect single-nucleotide crosslink sites in my CLIP data" -> Identify the exact base where the protein-RNA UV adduct caused the reverse transcriptase to stop (truncation, in iCLIP/eCLIP), to read through with a mutation (deletion in HITS-CLIP, T->C in PAR-CLIP), or to leave a multi-base signature (PARalyzer kernel density for PAR-CLIP). Single-nucleotide resolution is the foundation of motif registration (mCross), allele-specific binding (BEAPR/ASPRIN), variant-effect prediction, and the most rigorous comparisons across CLIP variants. The detection chemistry differs by CLIP type: iCLIP/eCLIP read 5' end is one nucleotide downstream of the crosslink (CITS); PAR-CLIP reads contain T->C transitions at the crosslink (CIMS substitution); HITS-CLIP reads contain deletions at the crosslink (CIMS deletion).

  • CLI (HMM, all CLIP variants): pureclip -i dedup.bam -bai dedup.bam.bai -g genome.fa -ibam sminput.bam -ibai sminput.bam.bai -o crosslinks.bed -or regions.bed -nt 8 -dm 8
  • CLI (CTK CITS truncations, iCLIP/eCLIP): parseAlignment.pl --map-qual 1 --min-len 18 --mutation-file mut.txt dedup.bam dedup.bed then tag2cluster.pl ... -cs5 5 -m 1 for truncation-cluster
  • CLI (CTK CIMS deletions, HITS-CLIP): getMutationType.pl dedup.bed mut.txt -type del then CIMS.pl dedup.bed mut.txt -big -c -p 0.01 cims.bed
  • CLI (CTK CIMS T->C, PAR-CLIP): getMutationType.pl dedup.bed mut.txt -type sub -nuc t -mut c then CIMS.pl dedup.bed t2c.mut -p 0.001 t2c_cims.bed
  • CLI (PAR-CLIP kernel density): PARalyzer params.ini (parameters file defines read length, min reads per cluster, mutation rate threshold)
  • CLI (PAR-CLIP wavClusteR R): wavClusteR::filterClusters(cl, snps=NULL, filterFC=FALSE) after wavelet clustering

Read the full file on GitHub · 260 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 7d ago First seen · 260 lines · 117 tokens per session scan A e4ddc2272c92

Subscribe to this mod's changes

bio-clip-seq-crosslink-site-detection is a skill published in the GitHub repository GPTomics/bioSkills (1,199 stars, last pushed 26d ago), licensed MIT. It adds 117 tokens to every session and 5,541 once invoked, about $0.0006 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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