tooluniverse-protein-sae-variant-interpretation

tooluniverse-protein-sae-variant-interpretation is a skill for Claude Code from mims-harvard/ToolUniverse. It costs 144 tokens per session (3,107 once invoked), scanned A, original, Apache-2.0.

A method for interpreting a single missense variant, meaning a DNA change that replaces one amino acid in a protein. It compares model representations of the normal and altered protein to identify biological features affected at the mutation site.

In plain words
What is it for?
Use it to compare reference and mutant protein representations and investigate why a specific amino-acid substitution might disrupt protein function.
Why use it?
It can provide a possible mechanism behind a damaging variant when a general pathogenicity score does not explain what changed. The result may show effects on catalytic sites, ligand binding, modifications, or structural motifs.

Skill for Claude Code

Written for Claude Code: disable-model-invocation in frontmatter.

Part of the tooluniverse plugin — 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server shipped together

Good fit Use it to compare reference and mutant protein representations and investigate why a specific amino-acid substitution might disrupt protein function.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/mims-harvard/tooluniverse/tooluniverse-protein-sae-variant-interpretation
About the project

ToolUniverse is a collection of tools, interfaces, and supporting components for building AI systems that perform scientific work. It is for developers creating AI scientist agents that use APIs, databases, machine-learning tools, and domain-specific utilities. The catalogue includes skills, commands, an MCP server, an agent, and a hook for working with the ecosystem.

mims-harvard/ToolUniverse · 1,680 stars · on GitHub · aiscientist.tools

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add mims-harvard/ToolUniverse --skill tooluniverse-protein-sae-variant-interpretation
Clone the repo
git clone --depth 1 https://github.com/mims-harvard/ToolUniverse

Made for: Claude Code.

Or install tooluniverse, the plugin that ships this one along with the rest of its 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for tooluniverse-protein-sae-variant-interpretation

README.md
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Your own site
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Your own site · 80×15
<a href="https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-protein-sae-variant-interpretation"><img src="https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-protein-sae-variant-interpretation.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 144 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 3,107 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00144 $0.03107
Opus 5 $0.00072 $0.01554
Sonnet 5 $0.00029 $0.00621
Haiku 4.5 $0.00014 $0.00311

Measured 8d ago against content hash 308365b4038c, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

tooluniverse-protein-sae-variant-interpretation scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 8d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

plugin/skills/tooluniverse-protein-sae-variant-interpretation/SKILL.md · 246 lines

How it starts

The opening of the file, as written. The whole thing — 246 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Protein SAE Variant Interpretation

Interpret a single missense variant by comparing reference vs mutant Sparse Autoencoder (SAE) feature activations from the ESMC-6B protein language model. SAE features are interpretable latent dimensions of the model's hidden state — many activate on biologically meaningful patterns (active sites, ligand-binding pockets, PTM sequons, structural motifs).


When to use this skill

Apply when users:

  • Ask "why is variant X (a missense) loss-of-function?" and need a mechanistic answer beyond a pathogenicity score
  • Have an AlphaMissense / ClinVar "damaging" variant and want to know which functional feature breaks (catalytic? binding? PTM site? structural?)
  • Want to compare ref vs mutant protein representation at a specific residue
  • Are interpreting why a structurally subtle change (single AA) has a big functional impact

Not for (use other skills instead):

  • ACMG pathogenicity classification → tooluniverse-variant-interpretation
  • Regulatory / non-coding variants → tooluniverse-variant-to-mechanism
  • Variant-to-disease association without mechanism → tooluniverse-gene-disease-association
  • Cancer-specific variant interpretation → tooluniverse-cancer-variant-interpretation

Required inputs

Input Format Example
Protein identifier UniProt accession or HGNC gene symbol P04637 or TP53
Variant Single-letter code: {ref_aa}{position_1idx}{alt_aa} R175H

Optional:

  • Window radius (default 8): residues around the mutation to analyze
  • Reference protein sequence (skip the UniProt lookup if already known)

Prerequisites

  • ESM_API_KEY env var with a valid EvolutionaryScale Forge token (https://forge.evolutionaryscale.ai)
  • esm package with SAE support:
    pip install 'esm @ git+https://github.com/evolutionaryscale/esm@ee891c52'
    
    The PyPI release of esm does NOT yet include SAEConfig. Install from the upstream feature branch.

License note: SAE outputs from Forge are governed by the Cambrian Inference Clickthrough License — non-commercial / academic research use only unless a separate commercial agreement applies.

Read the full file on GitHub · 246 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 8d ago First seen · 246 lines · 144 tokens per session scan A 308365b4038c

Subscribe to this mod's changes

tooluniverse-protein-sae-variant-interpretation is a skill published in the GitHub repository mims-harvard/ToolUniverse (1,680 stars, last pushed 3d ago), licensed Apache-2.0. It adds 144 tokens to every session and 3,107 once invoked, about $0.0007 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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