single-cell-multi-omics-data-harmonization

single-cell-multi-omics-data-harmonization is a skill for Claude Code, Codex from PharMolix/OpenBioMed. It costs 0 tokens per session (2,929 once invoked), scanned A, original, MIT.

A preparation workflow for combining RNA, protein, DNA-methylation, ATAC-seq, and other omics data, meaning measurements of different biological layers. It normalizes each data type, aligns feature names, corrects batch effects, and handles missing values.

In plain words
What is it for?
Use it to build a shared multi-omics container, normalize assay layers, inspect and correct batch structure, map identifiers to gene symbols, and filter or fill missing values.
Why use it?
Different assays use different scales, identifiers, and missing-data patterns, so they cannot be combined safely without preparation. This workflow creates consistently formatted data for later integration methods.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to build a shared multi-omics container, normalize assay layers, inspect and correct batch structure, map identifiers to gene symbols, and filter or fill missing values.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization
About the project

OpenBioMed is an agent platform and toolkit collection for biomedical research and drug discovery, covering areas such as molecular design, protein analysis, and single-cell data analysis. It is intended for researchers and provides the biomedical skills listed in the catalogue as workflows for Claude Code.

PharMolix/OpenBioMed · 1,106 stars · on GitHub

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PharMolix/OpenBioMed --skill single-cell-multi-omics-data-harmonization
Clone the repo
git clone --depth 1 https://github.com/PharMolix/OpenBioMed

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for single-cell-multi-omics-data-harmonization

README.md
[![agentmods](https://agentmods.dev/badge/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization/github.svg)](https://agentmods.dev/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization)
Your own site
<a href="https://agentmods.dev/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization"><img src="https://agentmods.dev/badge/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for single-cell-multi-omics-data-harmonization

Your own site · 80×15
<a href="https://agentmods.dev/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization"><img src="https://agentmods.dev/badge/skills/pharmolix/openbiomed/single-cell-multi-omics-data-harmonization.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 0 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,929 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector warn 7 Sept 2026
SkillSpector: 2 findings, up to medium

These are SkillSpector’s own severities. On a checked sample its high-severity flags on skills were ~96% false positives — a documented command, a public API, a “never do X” rule — so we show them as a caution to read, not a verdict. Why →

  • medium analysis-evasion · line 1
    Suspicious Unicode normalization or mixed-script content
    Fix: Review the flagged content for security risks. Ensure no credentials, secrets, or sensitive data are exposed.
  • medium Excessive Agency · line 35
    Skill enables autonomous high-impact decisions without human-in-the-loop verification. Critical operations (destructive commands, financial transactions, data deletion) should require explicit user confirmation.
    Fix: Add human-in-the-loop confirmation for destructive, irreversible, or high-impact operations. Never auto-execute commands that modify files, send data, or alter system state.
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00000 $0.02929
Opus 5 $0.00000 $0.01465
Sonnet 5 $0.00000 $0.00586
Haiku 4.5 $0.00000 $0.00293

Measured 9d ago against content hash 9b3a732c427b, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-08, from the pricing page.

Security

Grade A, and why

single-cell-multi-omics-data-harmonization scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

skills/single-cell-multi-omics-data-harmonization/SKILL.md · 261 lines

How it starts

The opening of the file, as written. The whole thing — 261 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Multi-Omics Data Harmonization

Prepare your RNA-seq, proteomics, methylation, and other omics datasets for joint integration by applying per-assay normalization, cross-assay batch correction, feature ID alignment, and missing value handling.

This is Step 1 of the multi-omics integration pipeline — all downstream skills (MOFA, DIABLO, SNF) depend on clean, consistently scaled output from this step.


What it does

  1. Loads each omics layer into a unified container (MultiAssayExperiment in R; MuData in Python)
  2. Applies the correct normalization strategy per data type:
    • RNA-seq counts → VST (DESeq2)
    • Proteomics LFQ intensity → log2 + median centering
    • Methylation β-values → M-value transformation: log2(β / 1−β)
    • ATAC-seq peaks → log1p(CPM); miRNA → log2(CPM + 1)
  3. Generates PCA plots before batch correction to visualize batch structure
  4. Applies ComBat batch correction across assays, preserving biological condition signal
  5. Generates PCA plots after correction to confirm batch removal
  6. Maps protein UniProt IDs and methylation probe IDs to HGNC gene symbols via Ensembl BioMart
  7. Filters features with > 30% missing values, then imputes remaining NAs with MinProb
  8. Z-scores all features and exports as .rds, .h5mu, and .csv for all downstream tools

Why this exists

If you ask a general AI to "prepare my multi-omics data for integration," it will:

  • Apply the same normalization to all data types (wrong — RNA counts need VST; methylation needs M-value transformation, not log2)
  • Run ComBat without checking for batch–condition confounding, silently removing biological signal
  • Skip feature ID alignment, leaving protein UniProt IDs unmapped to gene symbols
  • Not generate PCA plots to verify batch correction worked
  • Export data in a format incompatible with MOFA, DIABLO, or SNF

This skill encodes the correct methodological decisions:

  • Uses VST for RNA, log2+median centering for protein, and M-value for methylation — each chosen for statistical properties of that data type
  • Checks table(Batch, Condition) before ComBat to detect confounding
  • Aligns all features to HGNC gene symbols via Ensembl BioMart for cross-omics compatibility
  • Filters high-missingness features before imputation to avoid noise amplification
  • Exports in both R (.rds) and Python (.h5mu) formats for full downstream flexibility

Read the full file on GitHub · 261 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 261 lines · 0 tokens per session scan A 9b3a732c427b

Subscribe to this mod's changes

single-cell-multi-omics-data-harmonization is a skill published in the GitHub repository PharMolix/OpenBioMed (1,106 stars, last pushed 1mo ago), licensed MIT. It costs nothing until one of its globs matches a file; then it loads 2,929 tokens. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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