pathml

A Python toolkit for analysing whole-slide pathology images, which are very large scans of tissue samples viewed under a microscope. It supports image preparation, cell detection, spatial analysis, and machine-learning workflows across many slide formats.

In plain words
What is it for?
Use it to process H&E-stained slides, segment and classify nuclei, build cell or tissue graphs, analyse CODEX and Vectra images, quantify marker expression, train models, and manage large datasets.
Why use it?
It brings common pathology-image tasks into one workflow, so researchers do not need to assemble separate tools for loading slides, finding cells, and analysing tissue structure. It also helps work with multiplex images that show several biological markers at once.

Skill for Claude CodeCodex

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

agentmods
npx agentmods add skills/synthetic-sciences/openscience/pathml
Any agent
npx skills add synthetic-sciences/openscience --skill pathml
Clone the repo
git clone --depth 1 https://github.com/synthetic-sciences/openscience

Made for: Claude Code, Codex.

Per session 69 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,506 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. Scan, not verified.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5 $0.00069 $0.01506
Opus 5 $0.00034 $0.00753
Sonnet 5 $0.00014 $0.00301
Haiku 4.5 $0.00007 $0.00151

Measured 2d ago against content hash 5febafb20a15, method: parsed. Prices are Anthropic first-party input rates as of 2026-08-30, from the pricing page.

Security

Grade A, and why

pathml scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 2d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

Copies of this mod

1 near-identical copy found in the catalogue:

  • pathml — 88% identical, 6 lines differ
backend/cli/skills/biology/pathml/SKILL.md · 166 lines

How it starts

The opening of the file, as written. The whole thing — 166 lines — stays where its author put it; the contents beside it link to each section on GitHub.

PathML

Overview

PathML is a comprehensive Python toolkit for computational pathology workflows, designed to facilitate machine learning and image analysis for whole-slide pathology images. The framework provides modular, composable tools for loading diverse slide formats, preprocessing images, constructing spatial graphs, training deep learning models, and analyzing multiparametric imaging data from technologies like CODEX and multiplex immunofluorescence.

When to Use This Skill

Apply this skill for:

  • Loading and processing whole-slide images (WSI) in various proprietary formats
  • Preprocessing H&E stained tissue images with stain normalization
  • Nucleus detection, segmentation, and classification workflows
  • Building cell and tissue graphs for spatial analysis
  • Training or deploying machine learning models (HoVer-Net, HACTNet) on pathology data
  • Analyzing multiparametric imaging (CODEX, Vectra, MERFISH) for spatial proteomics
  • Quantifying marker expression from multiplex immunofluorescence
  • Managing large-scale pathology datasets with HDF5 storage
  • Tile-based analysis and stitching operations

Core Capabilities

PathML provides six major capability areas documented in detail within reference files:

1. Image Loading & Formats

Load whole-slide images from 160+ proprietary formats including Aperio SVS, Hamamatsu NDPI, Leica SCN, Zeiss ZVI, DICOM, and OME-TIFF. PathML automatically handles vendor-specific formats and provides unified interfaces for accessing image pyramids, metadata, and regions of interest.

See: references/image_loading.md for supported formats, loading strategies, and working with different slide types.

2. Preprocessing Pipelines

Build modular preprocessing pipelines by composing transforms for image manipulation, quality control, stain normalization, tissue detection, and mask operations. PathML's Pipeline architecture enables reproducible, scalable preprocessing across large datasets.

Key transforms:

  • StainNormalizationHE - Macenko/Vahadane stain normalization
  • TissueDetectionHE, NucleusDetectionHE - Tissue/nucleus segmentation
  • MedianBlur, GaussianBlur - Noise reduction
  • LabelArtifactTileHE - Quality control for artifacts

Read the full file on GitHub · 166 lines

Files

What ships with it

6 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 2d ago First seen · 166 lines · 69 tokens per session scan A 5febafb20a15

Subscribe to this mod's changes

pathml is a skill published in the GitHub repository synthetic-sciences/openscience (3,362 stars, last pushed 2d ago), licensed Apache-2.0. It adds 69 tokens to every session and 1,506 once invoked, about $0.0003 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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