Getting it into your agent
One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.
npx agentmods add skills/thesecondfox/skill/bio-sequence-manipulation-reverse-complementnpx skills add thesecondfox/skill --skill bio-sequence-manipulation-reverse-complementgit clone --depth 1 https://github.com/thesecondfox/skillWrote this? Show the measurements
A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.
[](https://agentmods.dev/skills/thesecondfox/skill/bio-sequence-manipulation-reverse-complement)<a href="https://agentmods.dev/skills/thesecondfox/skill/bio-sequence-manipulation-reverse-complement"><img src="https://agentmods.dev/badge/skills/thesecondfox/skill/bio-sequence-manipulation-reverse-complement.svg" alt="Measured on agentmods" height="20"></a>What it costs to keep this loaded
Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.
| Model | Per session | Once invoked |
|---|---|---|
| Fable 5.1 | $0.00039 | $0.01776 |
| Opus 5 | $0.00019 | $0.00888 |
| Sonnet 5 | $0.00008 | $0.00355 |
| Haiku 4.5 | $0.00004 | $0.00178 |
Grade A, and why
bio-reverse-complement scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 2d ago.
A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.
Nothing flagged
None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.
How it starts
The opening of the file, as written. The whole thing — 251 lines — stays where its author put it; the contents beside it link to each section on GitHub.
Version Compatibility
Reference examples tested with: BioPython 1.83+, samtools 1.19+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
Reverse Complement
Generate complementary and reverse complementary sequences using Biopython.
"Get the reverse complement" → Produce the 5'-to-3' sequence of the opposite strand.
- Python:
seq.reverse_complement()(BioPythonSeq) - CLI:
samtools faidx ref.fa region --reverse-complement
Required Import
from Bio.Seq import Seq
Core Methods
reverse_complement()
Returns the reverse complement (5' to 3' of the opposite strand).
seq = Seq('ATGCGATCG')
rc = seq.reverse_complement() # Returns Seq('CGATCGCAT')
This is the most commonly used operation - it gives you the sequence of the opposite strand in the conventional 5' to 3' direction.
complement()
Returns the complement without reversing.
seq = Seq('ATGCGATCG')
comp = seq.complement() # Returns Seq('TACGCTAGC')
Less commonly used - gives the opposite strand but in 3' to 5' direction.
reverse_complement_rna()
For RNA sequences (uses U instead of T):
rna = Seq('AUGCGAUCG')
rc_rna = rna.reverse_complement_rna() # Returns Seq('CGAUCGCAU')
complement_rna()
rna = Seq('AUGCGAUCG')
comp_rna = rna.complement_rna() # Returns Seq('UACGCUAGC')
Base Pairing Rules
DNA
| Base | Complement |
|---|---|
| A | T |
| T | A |
| G | C |
| C | G |
RNA
| Base | Complement |
|---|---|
| A | U |
| U | A |
| G | C |
| C | G |
Ambiguous Bases (IUPAC)
| Code | Bases | Complement |
|---|---|---|
| R | A/G | Y |
| Y | C/T | R |
| S | G/C | S |
| W | A/T | W |
| K | G/T | M |
| M | A/C | K |
| B | C/G/T | V |
| D | A/G/T | H |
| H | A/C/T | D |
| V | A/C/G | B |
| N | A/C/G/T | N |
What ships with it
1 file beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.
What this file has done since we first saw it
Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.
- 2d ago First seen · 251 lines · 39 tokens per session scan A 7e1b2b57a627
bio-reverse-complement is a skill published in the GitHub repository thesecondfox/skill (3 stars, last pushed 5mo ago), licensed MIT. It adds 39 tokens to every session and 1,776 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.
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