bio-annotation

Bio Annotation

Functional annotation and taxonomy inference from sequence homology.

Instructions

1. Read docs/README.md and the relevant tool guides before running anything.

2. Normalize tool outputs and generate the complete comparison bundle with the schema-backed driver:

   uv run --script skills/bio-annotation/scripts/build_annotation_artifacts.py \
     raw_annotations.tsv --genomes genomes.tsv --markers marker_catalog.tsv \
     --out results/bio-annotation
   

   The driver refuses a non-empty destination, enforces globally unique protein identifiers, writes normalized Parquet tables, adds explicit absent-marker rows, and computes query-specific/missing/expanded/contracted families against the reference median. The artifact contract is in schemas/artifacts.schema.json.

3. When a nucleotide assembly, MAG, genome, or contig FASTA is available, run /tracking-taxonomy-updates first for the BBTools-container QuickClade percontig domain screen. Use that routing table to choose the right taxonomy/QC path before interpreting protein annotations.

4. For InterProScan, read docs/interproscan-usage.md and validate the exact CLI with --help or --version. Current stable is v5.77-108.0; InterProScan 6 (Nextflow-based) is a forward-looking migration target.

5. Run InterProScan for domain/family annotation.

6. Run eggNOG-mapper v2.1.13+ for orthology-based annotation.

7. Run sequence-vs-database search and resolve taxonomy with TaxonKit v0.20.0+ (required for the March 2025 NCBI rank update that replaces "superkingdom" with "domain" and adds "realm" for viruses). Backend choice (DIAMOND, clustered nr, MMseqs2-GPU): see docs/README.md.

8. For domain-specific taxonomy after QuickClade:

   • Bacteria/Archaea -> run GTDB-Tk when genome/MAG-level sequence is available and cross-check NCBI/DIAMOND lineage assignments.
   • Viral/phage -> route to /bio-viromics; use PHROG/NCVOG markers and vConTACT3 only for phage/prokaryotic-virus contexts.
   • Giant-virus/Nucleocytoviricota -> route to /bio-viromics with GVClass and NCLDV marker-gene phylogeny.
   • Eukaryota -> use EukCC for MAG/genome QC and lineage context; avoid CheckM/GTDB-Tk assumptions.

9. For group-appropriate marker families, run HMM searches against the relevant profile libraries (Pfam, TIGRFAM, COG/arCOG, PHROG/NCVOG for viruses, eukaryotic ribosomal/structural HMMs when applicable). Use pyhmmer (Python bindings around HMMER 3.4 with native SIMD and batch-friendly APIs) by default; fall back to the HMMER CLI (hmmsearch / hmmscan) when an upstream tool requires it. The choice of profile libraries is derived from the literature-derived playbook for the inferred group.

10. Build an annotation-wide feature inventory by genome/contig and by gene family/domain/pathway.

11. Marker-gene census — from the literature-derived playbook, list the diagnostic marker / machinery categories for the inferred group (e.g., replication, transcription, translation-related such as ribosomal proteins and translation factors, packaging, capsid/structural, chromatin/SMC/topoisomerase, host-interaction). For EACH query genome and each comparison-set genome supplied, record presence and copy number per category. Save as marker_census.tsv (columns: genome, category, family_id, family_name, copy_number, evidence_source, e_value, notes). Expected-but-absent markers are first-class rows, not silent omissions.

12. Per-family copy-number matrix — build a Pfam/InterPro/HMM-family × genome integer matrix covering queries AND the supplied relatives. Persist as family_copy_number_matrix.parquet. Compute per-family fold change vs the relative median; flag query-specific families, missing-expected families, expansions, and contractions in family_expansion_candidates.tsv.

13. For exploratory work, read the literature-derived analysis playbook for the inferred organism or virus group before deciding what to flag.

14. Mine the inventory for discovery candidates relative to that playbook: expected features, missing expected features, rare or expanded families, unusual combinations, annotation/taxonomy conflicts, and high-value unknowns.

15. For specialized inputs such as viruses, organelles, symbionts, pathogens, or poorly characterized lineages, use the feature classes and outlier dimensions reported in the relevant literature rather than a fixed global checklist.

16. query_specific requires the family to be absent from every reference. When the reference median is 0 but at least one reference carries the family, the status is present_in_reference_minority with an empty fold_change: report it as a signal, never as a discovery.

17. Order discovery_candidates.tsv deterministically before reporting. Sort by status in the order query_specific, missing_expected, expanded, contracted, present_in_reference_minority; then by fold_change descending, with inf first and blank or non-numeric values last; then by genome and family_id ascending. Report the top rows in that order and keep the full table.