bedpe-format-handling

bedpe-format-handling is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 73 tokens per session (1,489 once invoked), scanned A, original, Apache-2.0.

A workflow for analysing paired-end ChIP-seq data stored in BEDPE files. ChIP-seq is a method for finding where a protein binds to DNA, and paired-end reads record both ends of each DNA fragment.

In plain words
What is it for?
Use it to process paired-end BEDPE files, estimate fragment length, and call protein-binding peaks, optionally comparing them with a control sample.
Why use it?
It keeps the two reads from each fragment together when estimating fragment length and finding binding regions. Treating them as separate reads can give incorrect results.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to process paired-end BEDPE files, estimate fragment length, and call protein-binding peaks, optionally comparing them with a control sample.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/bedpe-format-handling
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill bedpe-format-handling
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

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agentmods badge for bedpe-format-handling

README.md
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Your own site · 80×15
<a href="https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/bedpe-format-handling"><img src="https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/bedpe-format-handling.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 73 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,489 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00073 $0.01489
Opus 5 $0.00036 $0.00745
Sonnet 5 $0.00015 $0.00298
Haiku 4.5 $0.00007 $0.00149

Measured 10d ago against content hash 17ad067ad8f8, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-10, from the pricing page.

Security

Grade A, and why

bedpe-format-handling scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 10d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/bedpe-format-handling/SKILL.md · 95 lines

How it starts

The opening of the file, as written. The whole thing — 95 lines — stays where its author put it; the contents beside it link to each section on GitHub.

bedpe-format-handling

Summary

Handle paired-end ChIP-Seq data in BEDPE format to preserve fragment pairing information through peak-calling workflows. BEDPE format encodes both read pairs as six-column tab-separated coordinates, enabling proper fragment length estimation and paired-end-aware pileup.

When to use

Your ChIP-Seq input is paired-end sequencing data stored in BEDPE format (e.g., CTCF_PE_ChIP_chr22_50k.bedpe.gz), and you need to estimate fragment length and call peaks while respecting the paired nature of the reads rather than treating them as independent single-end alignments.

When NOT to use

  • Input is single-end ChIP-Seq data (use BED format instead with manual fragment length specification via --extsize)
  • Fragment length is already known and fixed across the experiment (use -f BED with explicit --extsize parameter for faster processing)
  • BEDPE file contains singletons or unpaired reads (filter or convert to BED first)

Inputs

  • BEDPE-format paired-end ChIP-Seq alignment file (gzip-compressed, e.g., .bedpe.gz)
  • Control (input) sample in BEDPE format (optional but recommended for peak calling)

Outputs

  • Fragment length estimate (d) in base pairs
  • narrowPeak file with peak coordinates and summit positions
  • bedGraph intermediate pileup tracks

How to apply

Pass the BEDPE file to macs3 predictd with the -f BEDPE flag to estimate average insertion length (fragment length d), which should be reported in base pairs (e.g., ~253 bp for the CTCF test data). Then run macs3 callpeak with -f BEDPE on the ChIP sample without specifying --extsize, allowing the pileup function to automatically handle paired-end fragment extent. Verify that the narrowPeak output file is generated with proper peak coordinates and summit positions. This workflow respects the original fragment boundaries encoded in the BEDPE coordinate pairs rather than requiring manual extension parameters.

Read the full file on GitHub · 95 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 10d ago First seen · 95 lines · 73 tokens per session scan A 17ad067ad8f8

Subscribe to this mod's changes

bedpe-format-handling is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed 4d ago), licensed Apache-2.0. It adds 73 tokens to every session and 1,489 once invoked, about $0.0004 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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