hi-c-fastq-to-contact-map-pipeline

hi-c-fastq-to-contact-map-pipeline is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 44 tokens per session (1,975 once invoked), scanned A, original, Apache-2.0.

A workflow that converts raw paired-end Hi-C sequencing reads into .hic contact-map files. It aligns reads to a reference genome, removes duplicate contacts, and builds the map using a standardized Juicer process.

In plain words
What is it for?
Use it for batch processing of new Hi-C sequencing data when you need kilobase-resolution maps following ENCODE-style processing standards.
Why use it?
Raw sequencing files do not directly show genome-region interactions. Processing them into contact maps makes the data ready for downstream three-dimensional genome analysis and reproducibility checks.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it for batch processing of new Hi-C sequencing data when you need kilobase-resolution maps following ENCODE-style processing standards.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/hi-c-fastq-to-contact-map-pipeline
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill hi-c-fastq-to-contact-map-pipeline
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

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README.md
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Per session 44 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,975 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00044 $0.01975
Opus 5 $0.00022 $0.00988
Sonnet 5 $0.00009 $0.00395
Haiku 4.5 $0.00004 $0.00198

Measured 9d ago against content hash a7b61c94de56, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

hi-c-fastq-to-contact-map-pipeline scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/hi-c-fastq-to-contact-map-pipeline/SKILL.md · 107 lines

How it starts

The opening of the file, as written. The whole thing — 107 lines — stays where its author put it; the contents beside it link to each section on GitHub.

hi-c-fastq-to-contact-map-pipeline

Summary

A unified workflow for converting raw Hi-C sequencing reads (FASTQ format) into contact maps (.hic binary files) via alignment, deduplication, and contact matrix construction. This skill enables reproducible Hi-C data processing following ENCODE uniform processing standards using the Juicer platform.

When to use

You have raw Hi-C FASTQ files from a sequencing experiment and need to generate kilobase-resolution Hi-C contact maps conforming to ENCODE reference standards. Use this when you need to validate pipeline reproducibility by comparing output checksums against reference outputs, or when integrating Hi-C processing into a larger genomic analysis workflow.

When NOT to use

  • Input is already a processed .hic or contact matrix file; use downstream analysis tools instead.
  • FASTQ files are from non-Hi-C protocols (e.g. standard RNA-seq, WGS); this pipeline is specific to Hi-C.
  • You require real-time or streaming processing; Juicer is designed for batch processing on clusters or cloud.

Inputs

  • Hi-C raw sequencing reads (FASTQ files, paired-end)
  • genome reference sequence (FASTA)
  • restriction site file (text, enzyme motifs)
  • chromosome sizes file (chrom.sizes format)

Outputs

  • Hi-C contact map (.hic binary format)
  • aligned and deduplicated read pairs (intermediate BAM/SAM files)
  • pipeline statistics and QC metrics (text/JSON)
  • output file checksum (MD5/SHA hash)

How to apply

Clone the ENCODE Hi-C uniform processing pipeline (encode_hic_pipeline) from ENCODE-DCC/hic-pipeline and configure Caper for your compute environment (local, cluster, or cloud). Prepare FASTQ input files in a designated directory and invoke the pipeline via caper run hic.wdl with a JSON configuration specifying genome ID, restriction enzyme site, and input paths. The pipeline performs read alignment via BWA, chimeric junction handling, deduplication, and contact matrix binning to produce a .hic binary file. Validate output correctness by computing file checksums (MD5 or SHA) and comparing against ENCODE reference checksums to confirm reproducibility.

Read the full file on GitHub · 107 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 107 lines · 44 tokens per session scan A a7b61c94de56

Subscribe to this mod's changes

hi-c-fastq-to-contact-map-pipeline is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 44 tokens to every session and 1,975 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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