narrow-peak-coordinate-validation

narrow-peak-coordinate-validation is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 50 tokens per session (1,391 once invoked), scanned A, original, Apache-2.0.

A validation step for narrowPeak files produced by MACS3 from paired-end ChIP-seq data. ChIP-seq identifies DNA regions associated with a selected protein, and narrow peaks represent focused binding regions.

In plain words
What is it for?
It is for verifying MACS3 peak-call output before using the regions in later ChIP-seq analysis.
Why use it?
It checks that peak coordinates and summit positions are present, correctly formatted, and consistent with the input fragment information.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit It is for verifying MACS3 peak-call output before using the regions in later ChIP-seq analysis.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/narrow-peak-coordinate-validation
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill narrow-peak-coordinate-validation
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for narrow-peak-coordinate-validation

README.md
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Your own site
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agentmods 80×15 button for narrow-peak-coordinate-validation

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Per session 50 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,391 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00050 $0.01391
Opus 5 $0.00025 $0.00696
Sonnet 5 $0.00010 $0.00278
Haiku 4.5 $0.00005 $0.00139

Measured 9d ago against content hash 231b224f9719, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

narrow-peak-coordinate-validation scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/narrow-peak-coordinate-validation/SKILL.md · 96 lines

How it starts

The opening of the file, as written. The whole thing — 96 lines — stays where its author put it; the contents beside it link to each section on GitHub.

narrow-peak-coordinate-validation

Summary

Verify that MACS3 callpeak correctly generates narrowPeak output files with valid peak coordinates and summit positions after processing paired-end ChIP-Seq data. This skill ensures the geometric correctness and completeness of peak call results before downstream analysis.

When to use

After running macs3 callpeak with the -f BEDPE flag on paired-end ChIP-Seq data (e.g., CTCF_PE_ChIP_chr22_50k.bedpe.gz), use this skill to validate that the narrowPeak output file exists and contains properly formatted peak coordinates and summit positions that align with the input fragment length estimates.

When NOT to use

  • Input is already validated narrowPeak output from a trusted prior run; re-validation adds no new information.
  • Single-end ChIP-Seq data where paired-end fragment length estimation is not applicable.
  • Broad peak calling mode (bdgbroadcall) instead of narrow peak mode; validation logic for peak width assumptions differs.

Inputs

  • narrowPeak file generated by macs3 callpeak
  • macs3 predictd fragment length estimate (e.g., ~253 bp)
  • Original paired-end bedpe input file metadata

Outputs

  • Validated narrowPeak coordinate set
  • Verification report of peak boundary correctness
  • Summit position validity confirmation

How to apply

Inspect the narrowPeak output file generated by macs3 callpeak to confirm three criteria: (1) the file is present and non-empty; (2) each peak record contains valid BED coordinates (chromosome, start, end) with start < end and within expected genomic ranges; (3) summit positions are recorded and fall within the peak interval boundaries. Cross-check that peak coordinates align with the fragment length estimate (e.g., ~253 bp for the CTCF tutorial) by verifying that peak widths and spacing are consistent with the estimated insertion length reported by macs3 predictd. This validation confirms that paired-end mode extension was correctly applied during pileup and peak calling without requiring manual --extsize specification.

Read the full file on GitHub · 96 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 96 lines · 50 tokens per session scan A 231b224f9719

Subscribe to this mod's changes

narrow-peak-coordinate-validation is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 50 tokens to every session and 1,391 once invoked, about $0.0003 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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