peak-calling-output-interpretation

peak-calling-output-interpretation is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 34 tokens per session (1,665 once invoked), scanned A, original, Apache-2.0.

A guide for checking and understanding BED output from peak-calling tools used with CUT&RUN data. Peak calling finds genomic regions with unusually strong signal, while BED is a text format that records genomic intervals and their measurements.

In plain words
What is it for?
Use it after a tool such as SEACR has produced BED peaks from sparse bedGraph signal. Review peak signal, genomic length, and related quality criteria before downstream analysis.
Why use it?
It explains what each output column means and helps distinguish credible peaks from regions affected by weak signal, excessive span, or other quality problems. It supports decisions about accepting, filtering, or changing thresholds.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one. Also seen: positional $N argument.

Good fit Use it after a tool such as SEACR has produced BED peaks from sparse bedGraph signal. Review peak signal, genomic length, and related quality criteria before downstream analysis.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/peak-calling-output-interpretation
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill peak-calling-output-interpretation
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

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agentmods badge for peak-calling-output-interpretation

README.md
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Per session 34 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,665 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00034 $0.01665
Opus 5 $0.00017 $0.00833
Sonnet 5 $0.00007 $0.00333
Haiku 4.5 $0.00003 $0.00167

Measured 6d ago against content hash ab7c6791a173, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

peak-calling-output-interpretation scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 6d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/peak-calling-output-interpretation/SKILL.md · 98 lines

How it starts

The opening of the file, as written. The whole thing — 98 lines — stays where its author put it; the contents beside it link to each section on GitHub.

peak-calling-output-interpretation

Summary

Interpret and validate the BED-format output from sparse peak-calling tools (e.g. SEACR) applied to CUT&RUN or chromatin profiling data, understanding the semantic meaning of each output field and assessing peak quality based on signal composition and genomic span.

When to use

You have run a peak-calling algorithm on sparse CUT&RUN bedGraph data and received a BED-format output file; you need to understand what each column represents, verify that the peaks meet biological and statistical criteria (total signal, max signal, span), and decide whether to accept, filter, or re-threshold the peak set.

When NOT to use

  • Input data is already a curated feature table or a manually annotated region set; peak calling and output interpretation are only needed when starting from raw or sparse bedGraph density.
  • Your analysis goal does not require peak-level interpretation; e.g., if you only need summary statistics (total number of peaks, genome coverage), a direct query of the BED file suffices.
  • Output is from a peak-calling tool with a different schema (e.g., narrowPeak with p-values and q-values); SEACR output lacks statistical significance estimates and uses signal-based metrics instead.

Inputs

  • SEACR output BED file (6-column: chr, start, end, total_signal, max_signal, max_signal_region)
  • Input bedGraph file (reference, for validation and cross-checking signal values)

Outputs

  • Validated or filtered peak set (BED format)
  • Quality report or metrics (peak count, signal distribution, pass/fail flags per peak)

How to apply

Open the output BED file (e.g., .stringent.bed or .relaxed.bed) and examine the six columns: chromosome, start, end, total signal, maximum bedgraph signal, and the coordinates of the maximum signal region. For each peak, verify that the total signal and max signal values are above the threshold used during calling (empirical control-based or numeric fractile). Check that the maximum signal region (field 6) falls within the peak coordinates (fields 2–3), indicating proper boundary detection. Cross-reference peaks against the input bedgraph to confirm that signal blocks were correctly merged and that zero-signal regions were properly omitted. Filter or flag peaks composed of very few input bedgraph lines (v1.2+ adds a line-count filter to remove artifacts from sparse composition). Use the choice of 'relaxed' versus 'stringent' mode (determined by the threshold applied: knee vs. peak of the total signal curve) to interpret expected sensitivity and specificity trade-offs.

Read the full file on GitHub · 98 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 6d ago First seen · 98 lines · 34 tokens per session scan A ab7c6791a173

Subscribe to this mod's changes

peak-calling-output-interpretation is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 34 tokens to every session and 1,665 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-06.

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