tooluniverse-enzyme-kinetics

tooluniverse-enzyme-kinetics is a skill for Claude Code from mims-harvard/ToolUniverse. It costs 123 tokens per session (1,518 once invoked), scanned A, original, Apache-2.0.

A data-analysis skill for measuring how quickly enzymes drive chemical reactions. It fits the Michaelis–Menten model to substrate and initial-velocity measurements and reports values such as Km, Vmax, kcat, catalytic efficiency, and inhibitor behavior.

In plain words
What is it for?
Use it to analyze your own enzyme experiments, estimate Km and Vmax, calculate turnover and catalytic efficiency, and classify competitive, uncompetitive, or non-competitive inhibition with Ki.
Why use it?
It turns reaction-rate measurements into standard enzyme constants and helps avoid misleading results from endpoint data or an unsuitable substrate range.

Skill for Claude Code

Written for Claude Code: disable-model-invocation in frontmatter.

Part of the tooluniverse plugin — 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server shipped together

Good fit Use it to analyze your own enzyme experiments, estimate Km and Vmax, calculate turnover and catalytic efficiency, and classify competitive, uncompetitive, or non-competitive inhibition with Ki.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics
About the project

ToolUniverse is a collection of tools, interfaces, and supporting components for building AI systems that perform scientific work. It is for developers creating AI scientist agents that use APIs, databases, machine-learning tools, and domain-specific utilities. The catalogue includes skills, commands, an MCP server, an agent, and a hook for working with the ecosystem.

mims-harvard/ToolUniverse · 1,680 stars · on GitHub · aiscientist.tools

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add mims-harvard/ToolUniverse --skill tooluniverse-enzyme-kinetics
Clone the repo
git clone --depth 1 https://github.com/mims-harvard/ToolUniverse

Made for: Claude Code.

Or install tooluniverse, the plugin that ships this one along with the rest of its 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for tooluniverse-enzyme-kinetics

README.md
[![agentmods](https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics/github.svg)](https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics)
Your own site
<a href="https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics"><img src="https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for tooluniverse-enzyme-kinetics

Your own site · 80×15
<a href="https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics"><img src="https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-enzyme-kinetics.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 123 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,518 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00123 $0.01518
Opus 5 $0.00062 $0.00759
Sonnet 5 $0.00025 $0.00304
Haiku 4.5 $0.00012 $0.00152

Measured 12d ago against content hash 5647a3e911fa, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-11, from the pricing page.

Security

Grade A, and why

tooluniverse-enzyme-kinetics scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 12d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (scripts/fit_michaelis_menten.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

plugin/skills/tooluniverse-enzyme-kinetics/SKILL.md · 91 lines

How it starts

The opening of the file, as written. The whole thing — 91 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Enzyme Kinetics (Michaelis-Menten)

Turn substrate concentration vs initial velocity data into Km, Vmax, kcat, and catalytic efficiency — and classify an inhibitor's mechanism.

The Michaelis-Menten model: v = Vmax·[S] / (Km + [S]).

When to use this

  • You measured initial reaction rates at several substrate concentrations.
  • You need Km (substrate affinity), Vmax, kcat (turnover number), or kcat/Km.
  • You have ±inhibitor velocity data and want to classify the inhibition mode + Ki.

For published kinetic constants (someone else's Km/kcat), use the BRENDA tools instead — this skill is for analyzing your own measured data.

Step 1 — Prepare the data

Issue What to do
Initial velocities, not endpoints v must be the initial rate (linear phase, <10% substrate consumed). Endpoint or plateaued rates give a wrong Km/Vmax.
Substrate range must span Km Include [S] both well below and well above Km (ideally ~0.2×Km to ~5×Km). Points only above Km can't define Km; only below can't define Vmax.
Units — be consistent One [S] unit (mM, µM) → Km comes back in that unit. One velocity unit. Keep them fixed.
For kcat you need [E] kcat = Vmax / [E]total. The tool's "catalytic_efficiency" is Vmax/Km on the velocity scale; to get true kcat (per-second turnover) and kcat/Km, divide Vmax by the molar enzyme concentration yourself.
≥5–7 points Few points → unstable fit. Spread them across the range, not clustered.

Step 2 — Fit Michaelis-Menten

tu run EnzymeKinetics_calculate '{"operation":"michaelis_menten",
  "substrate_concs":[0.1,0.25,0.5,1,2,5,10],
  "velocities":[8.5,18,32,52,72,90,98]}'

Returns a nonlinear_fit block (Vmax, Km, R2, SSE) — use these as the answer, a lineweaver_burk block (for reference only), catalytic_efficiency (Vmax/Km), and predicted_velocities + residuals.

Prefer the nonlinear fit, not Lineweaver-Burk. The double-reciprocal (Lineweaver-Burk) linearization distorts error (it over-weights low-[S] points) and is only for visualization/sanity — never report its Km/Vmax as the final values. The tool gives both; cite nonlinear_fit.

Read the full file on GitHub · 91 lines

Files

What ships with it

1 file beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 12d ago First seen · 91 lines · 123 tokens per session scan A 5647a3e911fa

Subscribe to this mod's changes

tooluniverse-enzyme-kinetics is a skill published in the GitHub repository mims-harvard/ToolUniverse (1,680 stars, last pushed 2d ago), licensed Apache-2.0. It adds 123 tokens to every session and 1,518 once invoked, about $0.0006 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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