by-epitope-analysis

by-epitope-analysis is a skill for Claude Code from 001TMF/blatant-why. It costs 6 tokens per session (4,522 once invoked), scanned A, original, MIT.

A structural-biology skill for finding and rating the part of a protein where a binder attaches. It selects important surface residues, assesses the shape and drug-development suitability of the binding site, and prepares inputs for design tools.

In plain words
What is it for?
Use it with a target-and-binder structure to select binding hotspots, classify the surface shape, assess suitability for biologic drugs, and refine design residues.
Why use it?
It turns structural information into a focused set of residues that a protein-design method can use.

Skill for Claude Code

Written for Claude Code: installed under .claude/.

Good fit Use it with a target-and-binder structure to select binding hotspots, classify the surface shape, assess suitability for biologic drugs, and refine design residues.

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Install with agentmods
npx agentmods add skills/001tmf/blatant-why/by-epitope-analysis
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add 001TMF/blatant-why --skill by-epitope-analysis
Clone the repo
git clone --depth 1 https://github.com/001TMF/blatant-why

Made for: Claude Code.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for by-epitope-analysis

README.md
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Your own site
<a href="https://agentmods.dev/skills/001tmf/blatant-why/by-epitope-analysis"><img src="https://agentmods.dev/badge/skills/001tmf/blatant-why/by-epitope-analysis/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for by-epitope-analysis

Your own site · 80×15
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Per session 6 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 4,522 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00006 $0.04522
Opus 5 $0.00003 $0.02261
Sonnet 5 $0.00001 $0.00904
Haiku 4.5 $0.00001 $0.00452

Measured 10d ago against content hash 76a0f1c79f7d, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-10, from the pricing page.

Security

Grade A, and why

by-epitope-analysis scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 10d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (scripts/select_hotspots.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

templates/.claude/skills/by-epitope-analysis/SKILL.md · 399 lines

How it starts

The opening of the file, as written. The whole thing — 399 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Skill: BY Epitope Analysis

You are an expert structural biologist performing epitope analysis and hotspot residue selection for protein and antibody binder design. This skill covers interface identification, residue classification, hotspot scoring, druggability assessment, and producing residue selections for PXDesign or BoltzGen input.


When to Use This Skill

Use this skill when you need to:

  • ✅ Convert a co-crystal structure into a hotspot residue list for PXDesign / BoltzGen
  • ✅ Classify and score interface residues from a target+binder PDB entry
  • ✅ Decide whether an epitope is concave/convex/flat and pick a design tool accordingly
  • ✅ Assess druggability of an epitope for biologics
  • ✅ Refine an epitope selection produced by by-research before committing to design

Don't use this skill for:

  • ❌ Sequence-only target analysis with no structure → use by-research to find a structure first
  • ❌ Picking PDB entries from a target name → use mcp__by-pdb__pdb_search directly
  • ❌ Predicting structures from sequence → use Protenix (see protenix skill)
  • ❌ Liability scanning (CDR loops, developability) → use by-screening

Quick Start

Run the hotspot selection script on a downloaded PDB with chain assignments:

python scripts/select_hotspots.py \
  --pdb /tmp/5JXE.cif \
  --target-chain A \
  --binder-chains H,L \
  --cutoff 5.0 \
  --top-n 6 \
  --out /tmp/hotspots.json

Expected output:

✓ Loaded structure 5JXE
✓ Interface residues detected: 18 (cutoff 5.0 A)
✓ Ranked 18 candidates; selected 6 hotspots
✓ Wrote /tmp/hotspots.json

The JSON contains a ranked list of {chain, resseq, resname, score, classification, rationale} plus a range_notation string ready to paste into BoltzGen entity YAML.


Installation

Software Version License Commercial Use Installation Command
Python ≥3.9 PSF ✅ Permitted preinstalled
BioPython ≥1.81 Biopython License ✅ Permitted pip install biopython
NumPy ≥1.24 BSD-3 ✅ Permitted pip install numpy

Read the full file on GitHub · 399 lines

Files

What ships with it

4 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 10d ago First seen · 399 lines · 6 tokens per session scan A 76a0f1c79f7d

Subscribe to this mod's changes

by-epitope-analysis is a skill published in the GitHub repository 001TMF/blatant-why (114 stars, last pushed 24d ago), licensed MIT. It adds 6 tokens to every session and 4,522 once invoked, about $0.0000 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.