wgcna-analysis

wgcna-analysis is a skill for Claude Code, Codex from aipoch/medical-research-skills. It costs 85 tokens per session (2,967 once invoked), scanned A, original, MIT.

A workflow for building a weighted gene co-expression network from bulk gene-expression data. It groups genes that change together into modules and compares those modules with sample traits or groups.

In plain words
What is it for?
Use it with a bulk expression matrix and sample group file to filter variable genes, identify co-expression modules, correlate them with traits, and export plots and gene tables.
Why use it?
Looking at genes one by one can hide coordinated biological patterns. Network modules make it easier to find groups of related genes associated with a trait.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Needs its repository: it runs a file that does not travel with it, so clone the repository first. The line is --output_dir ./output/ \.

Good fit Use it with a bulk expression matrix and sample group file to filter variable genes, identify co-expression modules, correlate them with traits, and export plots and gene tables.

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About the project

Medical Research Agent Skills is a library of agent instructions for medical and biomedical research, covering evidence analysis, study protocol design, data analysis, and academic writing. Researchers use it to guide compatible coding agents through common scientific workflows. The catalogue contains many of the library's skills and commands.

aipoch/medical-research-skills · 1,860 stars · on GitHub · aipoch.com

Install

Getting it into your agent

It runs from inside its repository, so the clone comes first — what it calls does not travel with the file alone.

Clone the repo
git clone --depth 1 https://github.com/aipoch/medical-research-skills
agentmods
npx agentmods add skills/aipoch/medical-research-skills/wgcna-analysis

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for wgcna-analysis

README.md
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Your own site
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Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for wgcna-analysis

Your own site · 80×15
<a href="https://agentmods.dev/skills/aipoch/medical-research-skills/wgcna-analysis"><img src="https://agentmods.dev/badge/skills/aipoch/medical-research-skills/wgcna-analysis.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 85 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,967 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00085 $0.02967
Opus 5 $0.00043 $0.01484
Sonnet 5 $0.00017 $0.00593
Haiku 4.5 $0.00009 $0.00297

Measured 9d ago against content hash 9b087bf97049, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

wgcna-analysis scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

awesome-med-research-skills/Data Analysis/wgcna-analysis/SKILL.md · 276 lines

How it starts

The opening of the file, as written. The whole thing — 276 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Source: https://github.com/aipoch/medical-research-skills

WGCNA Analysis

When to Read External Files

AI Agent: This section tells you when to read additional files.

Situation File to Read Purpose
Need algorithm details references/algorithm.md WGCNA workflow, filtering strategy, statistics, assumptions
Need to run analysis scripts/main.R Execute: Rscript scripts/main.R --input_file ... --group_file ...
Encounter errors references/troubleshooting.md Common errors, causes, and fixes
Need CLI examples references/cli-guide.md Complete command examples for common scenarios
Need conversion audit details references/diagnosis-report.md Skill readiness assessment and remediation summary
Need test data tests/data/ Minimal example input files for validation

When Not to Use

  • Do not use for single-cell RNA-seq matrices.
  • Do not use for methylation data, DEG testing, or other non-WGCNA workflows.
  • Do not use if the user only wants exploratory discussion and does not want the analysis executed.
  • Do not proceed if the dataset is obviously too small for WGCNA or becomes too small after QC.

When an input is out of scope, stop early and state which limitation applies.


Usage

Rscript scripts/main.R \
  --input_file ./expression_matrix.csv \
  --group_file ./group_info.csv \
  --output_dir ./output/ \
  --sample_column sample \
  --group_column group \
  --network_type unsigned \
  --cor_type pearson \
  --mad_quantile 0.25 \
  --min_mad 0.01 \
  --max_genes 5000 \
  --min_module_size 30 \
  --merge_cut_height 0.25 \
  --soft_r2_cutoff 0.85 \
  --module_of_interest auto \
  --top_modules 1 \
  --tom_sample_size 400 \
  --chunk_size 0 \
  --seed 42 \
  --timeout_seconds 0

Arguments

Short Long Type Default Description
-i --input_file character required Expression matrix file with genes in rows and samples in columns
-g --group_file character required Sample-to-group mapping file
-o --output_dir character ./output/ Output directory
-a --sample_column character sample Sample column in the group file; falls back to the first column if not found
-b --group_column character group Group column in the group file; falls back to the second column if not found
-n --network_type character unsigned Network type for WGCNA: unsigned or signed
-c --cor_type character pearson Correlation type: pearson or bicor
-q --mad_quantile double 0.25 MAD quantile used to define the variability cutoff
-m --min_mad double 0.01 Minimum MAD cutoff combined with the quantile filter
-k --max_genes integer 0 Maximum number of retained variable genes; 0 keeps all filtered genes
-p --min_module_size integer 30 Minimum module size used by blockwiseModules()
-r --merge_cut_height double 0.25 Merge cut height for module merging
-u --soft_r2_cutoff double 0.85 Target scale-free topology R-squared cutoff for soft-threshold selection
-t --trait_of_interest character NULL Trait column used for module membership vs trait scatter plots; defaults to the first trait column
-x --module_of_interest character auto Module color or comma-separated module colors to export; auto ranks modules by absolute module-trait correlation
--top_modules integer 1 Number of top-ranked modules to export when module_of_interest=auto
-y --tom_sample_size integer 400 Number of genes sampled for the TOM heatmap
--chunk_size integer 0 Row chunk size for large expression matrices; 0 disables chunked loading
-s --seed integer 42 Random seed for reproducibility and TOM heatmap sampling
-z --timeout_seconds integer 0 Optional elapsed-time limit in seconds; 0 disables timeout

Read the full file on GitHub · 276 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 276 lines · 85 tokens per session scan A 9b087bf97049

Subscribe to this mod's changes

wgcna-analysis is a skill published in the GitHub repository aipoch/medical-research-skills (1,860 stars, last pushed 1mo ago), licensed MIT. It adds 85 tokens to every session and 2,967 once invoked, about $0.0004 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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