cpg-base-filtering-by-statistical-threshold

cpg-base-filtering-by-statistical-threshold is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 42 tokens per session (1,450 once invoked), scanned A, original, Apache-2.0.

A statistical filtering step for selecting important CpG methylation changes from a tested dataset. CpG sites are DNA positions where a cytosine is followed by a guanine.

In plain words
What is it for?
Use it after differential methylation testing to separate high-confidence increases and decreases for later annotation or validation.
Why use it?
It reduces a large set of tested sites to changes that meet both a statistical-significance cutoff and a minimum methylation difference.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it after differential methylation testing to separate high-confidence increases and decreases for later annotation or validation.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/cpg-base-filtering-by-statistical-threshold
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill cpg-base-filtering-by-statistical-threshold
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

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README.md
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Per session 42 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,450 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00042 $0.01450
Opus 5 $0.00021 $0.00725
Sonnet 5 $0.00008 $0.00290
Haiku 4.5 $0.00004 $0.00145

Measured 9d ago against content hash 267435655c38, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

cpg-base-filtering-by-statistical-threshold scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/cpg-base-filtering-by-statistical-threshold/SKILL.md · 95 lines

How it starts

The opening of the file, as written. The whole thing — 95 lines — stays where its author put it; the contents beside it link to each section on GitHub.

CpG base filtering by statistical threshold

Summary

Filter differentially methylated CpG bases from a methylDiff object using combined q-value and percent methylation difference thresholds to isolate statistically significant and biologically meaningful changes. This skill extracts hyper- and hypo-methylated base sets for downstream annotation and validation.

When to use

After calculateDiffMeth() has been run on a methylBase object and you have a methylDiff object with q-values and methylation difference estimates. Apply this skill when you need to reduce the set of all tested CpG bases to a high-confidence subset meeting both statistical significance (q-value < 0.01) and minimum effect size (≥25% methylation difference) criteria for validation or annotation.

When NOT to use

  • Input is a raw methylation call file or methylRawList object that has not yet been merged and tested for differential methylation — use unite() and calculateDiffMeth() first.
  • You have already applied sample-level filtering (coverage, PCR bias) but have not yet merged samples — call unite() before differential methylation testing.
  • The analysis goal requires region-level rather than base-pair-level differential methylation — use regional or tiling window analysis functions instead.

Inputs

  • methylDiff object (output from calculateDiffMeth with q-values and percent methylation differences already computed)

Outputs

  • methylDiff object with hyper-methylated bases (type='hyper')
  • methylDiff object with hypo-methylated bases (type='hypo')

How to apply

Call getMethylDiff() on a methylDiff object with q-value threshold of 0.01 and percent methylation difference cutoff of 25%, specifying type='hyper' or type='hypo' to extract separate base objects for each direction of change. The q-value threshold controls false discovery rate from Fisher's exact test or logistic regression (automatically selected based on sample size in calculateDiffMeth), while the 25% methylation difference threshold ensures only bases with substantial changes in methylation percentage are retained. Extract hyper-methylated bases (increased methylation) and hypo-methylated bases (decreased methylation) as separate methylDiff objects for parallel validation. Validate output by confirming the resulting hyper and hypo base counts match expected values from prior runs or published vignettes.

Read the full file on GitHub · 95 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 95 lines · 42 tokens per session scan A 267435655c38

Subscribe to this mod's changes

cpg-base-filtering-by-statistical-threshold is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 42 tokens to every session and 1,450 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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