nucleotide-footprint-pattern-recognition

nucleotide-footprint-pattern-recognition is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 45 tokens per session (2,092 once invoked), scanned A, original, Apache-2.0.

A method for finding signs that a transcription factor is actually bound to DNA in ATAC-seq data. ATAC-seq measures open DNA, and a footprint is a small protected or less-accessible area left where a bound protein blocks the sequencing enzyme.

In plain words
What is it for?
It is for measuring transcription-factor occupancy across the genome and comparing binding changes between conditions.
Why use it?
A DNA sequence motif only shows where a protein could bind; footprint analysis helps distinguish possible sites from sites with evidence of occupancy.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit It is for measuring transcription-factor occupancy across the genome and comparing binding changes between conditions.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/nucleotide-footprint-pattern-recognition
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill nucleotide-footprint-pattern-recognition
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for nucleotide-footprint-pattern-recognition

README.md
[![agentmods](https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/nucleotide-footprint-pattern-recognition/github.svg)](https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/nucleotide-footprint-pattern-recognition)
Your own site
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agentmods 80×15 button for nucleotide-footprint-pattern-recognition

Your own site · 80×15
<a href="https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/nucleotide-footprint-pattern-recognition"><img src="https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/nucleotide-footprint-pattern-recognition.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 45 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,092 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector pass 7 Sept 2026
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00045 $0.02092
Opus 5 $0.00023 $0.01046
Sonnet 5 $0.00009 $0.00418
Haiku 4.5 $0.00005 $0.00209

Measured 9d ago against content hash 7c5166276e77, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

nucleotide-footprint-pattern-recognition scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/nucleotide-footprint-pattern-recognition/SKILL.md · 110 lines

How it starts

The opening of the file, as written. The whole thing — 110 lines — stays where its author put it; the contents beside it link to each section on GitHub.

nucleotide-footprint-pattern-recognition

Summary

Identify and quantify transcription factor occupancy by detecting the characteristic depletion of Tn5 insertion signals around protein-bound DNA motifs in ATAC-seq data. This skill reveals footprints—localized regions of accessibility reduction caused by protein binding—which distinguish occupied from unoccupied transcription factor binding sites.

When to use

Apply this skill when you have aligned ATAC-seq BAM files and want to discriminate between transcription factor binding sites that are actually occupied by protein versus sites with matching sequence motifs that are unbound. Use it when your research question requires quantifying the extent of transcription factor occupancy genome-wide or identifying condition-specific changes in binding kinetics across regulatory regions.

When NOT to use

  • Input ATAC-seq data is not from bulk chromatin or has insufficient sequencing depth (<10 million reads); low coverage compromises the statistical power to detect footprints. Single-cell data requires aggregation into pseudobulk BAM files per cell cluster first.
  • Transcription factor motif coordinates are unavailable or of poor quality; footprinting requires known or predicted binding site locations to anchor the analysis window.
  • Your primary goal is to identify novel transcription factor binding sites de novo rather than to quantify occupancy at known motif locations; footprinting detects occupancy signal but does not perform motif discovery.

Inputs

  • ATAC-seq aligned reads (BAM format)
  • Reference genome sequence (FASTA)
  • Transcription factor motif coordinate annotations (BED format with classified bound/unbound status or accessibility signal values for classification)
  • Open chromatin peak coordinates (BED format, optional but recommended for ATACorrect)
  • Transcription factor position weight matrices or motif annotations

Outputs

  • Bias-corrected ATAC-seq cut-site signal (BigWig format)
  • Footprint score matrix (insertion counts by genomic position × site class)
  • Positional insertion distribution statistics (mean and standard deviation per bin)
  • Aggregate footprint visualization (plot showing insertion profiles for bound vs. unbound sites)
  • Bound/unbound classification confidence scores or differential binding estimates

Read the full file on GitHub · 110 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 110 lines · 45 tokens per session scan A 7c5166276e77

Subscribe to this mod's changes

nucleotide-footprint-pattern-recognition is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 45 tokens to every session and 2,092 once invoked, about $0.0002 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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