tooluniverse-crispr-screen-analysis

tooluniverse-crispr-screen-analysis is a skill for Claude Code from mims-harvard/ToolUniverse. It costs 83 tokens per session (2,712 once invoked), scanned A, original, Apache-2.0.

A workflow for analyzing CRISPR-Cas9 gene screens, which test how changing genes affects cell growth or survival. It examines guide-RNA quality, compares repeated experiments, ranks important genes, and groups results into biological pathways.

In plain words
What is it for?
Use it to study genes needed for cell survival, find synthetic-lethal gene pairs, identify possible drug targets, and compare dropout or positive-selection screens. It can also find biological pathways enriched among the strongest results.
Why use it?
It helps turn large screen results into reliable gene-level findings without manually checking every guide or experiment. It also helps distinguish likely biological hits from noisy data.

Skill for Claude Code

Written for Claude Code: disable-model-invocation in frontmatter.

Part of the tooluniverse plugin — 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server shipped together

Good fit Use it to study genes needed for cell survival, find synthetic-lethal gene pairs, identify possible drug targets, and compare dropout or positive-selection screens. It can also find biological pathways enriched among the strongest results.

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Install with agentmods
npx agentmods add skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis
About the project

ToolUniverse is a collection of tools, interfaces, and supporting components for building AI systems that perform scientific work. It is for developers creating AI scientist agents that use APIs, databases, machine-learning tools, and domain-specific utilities. The catalogue includes skills, commands, an MCP server, an agent, and a hook for working with the ecosystem.

mims-harvard/ToolUniverse · 1,680 stars · on GitHub · aiscientist.tools

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add mims-harvard/ToolUniverse --skill tooluniverse-crispr-screen-analysis
Clone the repo
git clone --depth 1 https://github.com/mims-harvard/ToolUniverse

Made for: Claude Code.

Or install tooluniverse, the plugin that ships this one along with the rest of its 140 skills, 8 commands, 1 agent, 1 hook, 1 MCP server.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for tooluniverse-crispr-screen-analysis

README.md
[![agentmods](https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis/github.svg)](https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis)
Your own site
<a href="https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis"><img src="https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for tooluniverse-crispr-screen-analysis

Your own site · 80×15
<a href="https://agentmods.dev/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis"><img src="https://agentmods.dev/badge/skills/mims-harvard/tooluniverse/tooluniverse-crispr-screen-analysis.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 83 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 2,712 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • Socket pass 29 Mar 2026
  • Snyk pass 29 Mar 2026
  • NVIDIA SkillSpector warn 7 Sept 2026
SkillSpector: 1 finding, up to medium

These are SkillSpector’s own severities. On a checked sample its high-severity flags on skills were ~96% false positives — a documented command, a public API, a “never do X” rule — so we show them as a caution to read, not a verdict. Why →

  • medium Excessive Agency · line 161
    Skill enables autonomous high-impact decisions without human-in-the-loop verification. Critical operations (destructive commands, financial transactions, data deletion) should require explicit user confirmation.
    Fix: Add human-in-the-loop confirmation for destructive, irreversible, or high-impact operations. Never auto-execute commands that modify files, send data, or alter system state.
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00083 $0.02712
Opus 5 $0.00042 $0.01356
Sonnet 5 $0.00017 $0.00542
Haiku 4.5 $0.00008 $0.00271

Measured 11d ago against content hash c3bbb208367f, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-11, from the pricing page.

Security

Grade A, and why

tooluniverse-crispr-screen-analysis scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 11d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (scripts/reference_gene_sets.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

plugin/skills/tooluniverse-crispr-screen-analysis/SKILL.md · 210 lines

How it starts

The opening of the file, as written. The whole thing — 210 lines — stays where its author put it; the contents beside it link to each section on GitHub.

ToolUniverse CRISPR Screen Analysis

RULE ZERO — Check for pre-computed results FIRST

Before following any instruction below, scan the data folder for:

  • *_executed.ipynb → read with tu run read_executed_notebook '{"data_folder":"<path>","search":"<keyword>"}' and cite its cell outputs as the authoritative answer
  • Pre-computed result files (CSV/TSV with names like *results*, *deseq*, *enrich*, *stats*, *_simplified.csv) → read directly and report the requested value
  • Canonical analysis scripts (analysis.R, run_*.py, find_*.R, *.Rmd) → execute as-is and read the output

Only follow this skill's re-analysis recipe below if none of the above exist. Re-running from raw data produces different numbers than the published answer and is much slower (often 5-10× turn count).


Comprehensive skill for analyzing CRISPR-Cas9 genetic screens to identify essential genes, synthetic lethal interactions, and therapeutic targets through robust statistical analysis and pathway enrichment.

Overview

CRISPR screens enable genome-wide functional genomics by systematically perturbing genes and measuring fitness effects. This skill provides an 8-phase workflow for:

  • Processing sgRNA count matrices
  • Quality control and normalization
  • Gene-level essentiality scoring (MAGeCK-like and BAGEL-like approaches)
  • Synthetic lethality detection
  • Pathway enrichment analysis
  • Drug target prioritization with DepMap integration
  • Integration with expression and mutation data

Core Workflow

Phase 1: Data Import & sgRNA Count Processing

Load sgRNA count matrix (MAGeCK format or generic TSV). Expected columns: sgRNA, Gene, plus sample columns. Create experimental design table linking samples to conditions (baseline/treatment) with replicate assignments.

Phase 2: Quality Control & Filtering

Assess sgRNA distribution quality:

  • Library sizes per sample (total reads)
  • Zero-count sgRNAs: Count across samples
  • Low-count filtering: Remove sgRNAs below threshold (default: <30 reads in >N-2 samples)
  • Gini coefficient: Assess distribution skewness per sample
  • Report filtering recommendations

Read the full file on GitHub · 210 lines

Files

What ships with it

8 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 11d ago First seen · 210 lines · 83 tokens per session scan A c3bbb208367f

Subscribe to this mod's changes

tooluniverse-crispr-screen-analysis is a skill published in the GitHub repository mims-harvard/ToolUniverse (1,680 stars, last pushed yesterday), licensed Apache-2.0. It adds 83 tokens to every session and 2,712 once invoked, about $0.0004 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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