bio-atac-seq-single-cell-atac

bio-atac-seq-single-cell-atac is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 123 tokens per session (6,366 once invoked), scanned A, a copy of bio-atac-seq-single-cell-atac, MIT.

A workflow guide for analyzing single-cell ATAC-seq data, which measures open regions of DNA in individual cells. It supports common tools such as Signac, ArchR, SnapATAC2, and Cell Ranger ATAC, including paired RNA-and-ATAC data.

In plain words
What is it for?
Use it to create per-cell quality checks, reduce dimensions, cluster cells, identify open DNA regions, assign cell types, and combine ATAC data with single-cell RNA data.
Why use it?
It helps choose and use compatible analysis tools while checking data quality, software versions, and results across many cells.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to create per-cell quality checks, reduce dimensions, cluster cells, identify open DNA regions, assign cell types, and combine ATAC data with single-cell RNA data.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PKU-YuanGroup/OpenAI4S --skill bio-atac-seq-single-cell-atac
Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-atac-seq-single-cell-atac

README.md
[![agentmods](https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac/github.svg)](https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac)
Your own site
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-atac-seq-single-cell-atac

Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-atac-seq-single-cell-atac.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 123 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 6,366 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin 98% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00123 $0.06366
Opus 5 $0.00062 $0.03183
Sonnet 5 $0.00025 $0.01273
Haiku 4.5 $0.00012 $0.00637

Measured 13d ago against content hash 96bb386937c4, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

bio-atac-seq-single-cell-atac scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 13d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

This is a copy

98% identical to bio-atac-seq-single-cell-atac — 12 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-atac-seq-single-cell-atac/SKILL.md · 413 lines

How it starts

The opening of the file, as written. The whole thing — 413 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: Cell Ranger ATAC 2.1+, Signac 1.13+, Seurat 5.0+, ArchR 1.0.2+, SnapATAC2 2.8+, AMULET 1.1+, scDblFinder 1.16+, scater 1.30+, scvi-tools 1.1+, GenomicRanges 1.54+, JASPAR2024 0.99+, BSgenome.Hsapiens.UCSC.hg38 1.4+, EnsDb.Hsapiens.v86 2.99+, MACS3 3.0+. SnapATAC2 2.8+ uses pp.import_fragments; older 2.5-2.7 used pp.import_data (renamed/removed in 2.9).

Verify before use:

  • Python: pip show <package> then help(module.function) to check signatures
  • R: packageVersion('<pkg>') then ?function_name to verify parameters
  • CLI: <tool> --version then <tool> --help to confirm flags

If code throws unexpected errors, introspect the installed package and adapt rather than retrying.

Single-Cell ATAC-seq

"Process my 10X scATAC data from cellranger output" -> Build a per-cell fragment matrix, compute per-cell QC, dimensionality reduction (TF-IDF + LSI / spectral / autoencoder), cluster, call cluster-level pseudobulk peaks, annotate cell types via gene-activity scores, and integrate with paired scRNA-seq if Multiome.

  • R: Signac::CreateChromatinAssay() -> Seurat workflow (TF-IDF + SVD + UMAP + Leiden)
  • R: ArchR::createArrowFiles() -> ArchR project (TileMatrix + LSI + UMAP)
  • Python: snapatac2.pp.import_fragments() -> SnapATAC2 (spectral / diffusion-map clustering)
  • CLI (preprocessing): cellranger-atac count (10X) or chromap (alignment-only fragment files)

Ecosystem Choice (The Most Important Decision)

Ecosystem Language Strength Fails when Best for
Signac (Stuart 2021) R, Seurat-based Tightest scRNA-seq integration; Seurat ecosystem mature Memory hungry on >100K cells; slower than ArchR Multiome RNA+ATAC; small-to-medium datasets; Seurat user
ArchR (Granja 2021) R, Arrow/HDF5 Memory-efficient (Arrow files); fast on 100K-1M cells; built-in trajectory + doublet Less RNA-seq integration; ArchR-specific format Large bulk-cohort scATAC; trajectory analysis; ATAC-only
SnapATAC2 (Zhang 2024) Python, AnnData Memory-efficient; modern Python ecosystem; spectral clustering performant Newer; benchmarks evolving; ecosystem smaller than R Python-first labs; very large datasets (>1M cells)
Cell Ranger ATAC CLI (10X-specific) 10X official preprocessing Closed; fixed pipeline Only as preprocessing step; analysis happens elsewhere
scATAC-pro CLI-based pipeline Alternative preprocessing Less maintained Legacy; not recommended for new projects

Read the full file on GitHub · 413 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 13d ago First seen · 413 lines · 123 tokens per session scan A 96bb386937c4

Subscribe to this mod's changes

bio-atac-seq-single-cell-atac is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 123 tokens to every session and 6,366 once invoked, about $0.0006 per session on Opus 5. A static security scan graded it A with 0 findings. It is 98% identical to bio-atac-seq-single-cell-atac, differing in 12 lines, and is treated as a copy.

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