bio-comparative-genomics-genome-distance-and-species-delineation

bio-comparative-genomics-genome-distance-and-species-delineation is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 218 tokens per session (7,579 once invoked), scanned A, a copy of bio-comparative-genomics-genome-distance-and-species-delineation, MIT.

A set of methods for measuring how genetically similar two genomes are and using those measurements for classification. It includes nucleotide and amino-acid identity, DNA hybridization estimates, and k-mer comparisons.

In plain words
What is it for?
Use it to calculate genome distances, compare bacterial or archaeal isolates, assign taxonomy, and check whether assemblies represent the same or different organisms.
Why use it?
It provides quantitative evidence for deciding whether organisms are closely related or should be treated as different species or taxonomic groups.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one. Also seen: positional $N argument.

Good fit Use it to calculate genome distances, compare bacterial or archaeal isolates, assign taxonomy, and check whether assemblies represent the same or different organisms.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PKU-YuanGroup/OpenAI4S --skill bio-comparative-genomics-genome-distance-and-species-delineation
Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-comparative-genomics-genome-distance-and-species-delineation

README.md
[![agentmods](https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation/github.svg)](https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation)
Your own site
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-comparative-genomics-genome-distance-and-species-delineation

Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-comparative-genomics-genome-distance-and-species-delineation.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 218 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 7,579 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 1 finding. A grade says what 26 rules found in the file — not that it is safe.
Origin 98% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00218 $0.07579
Opus 5 $0.00109 $0.03789
Sonnet 5 $0.00044 $0.01516
Haiku 4.5 $0.00022 $0.00758

Measured 9d ago against content hash 1ae051460853, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

bio-comparative-genomics-genome-distance-and-species-delineation scanned grade A with 1 finding against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (scripts/skani_ani_species_delineation.sh), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Makes network callslowCapability

Not a fault in itself. Listed so you know the mod talks to something, and to what.

wget https://data.gtdb.ecogenomic.org/releases/release220/220.0/auxillary_files/gtdbtk_r220_data.tar.gz
Origin

This is a copy

98% identical to bio-comparative-genomics-genome-distance-and-species-delineation — 14 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-comparative-genomics-genome-distance-and-species-delineation/SKILL.md · 446 lines

How it starts

The opening of the file, as written. The whole thing — 446 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: skani 0.2.5+ (Shaw & Yu 2023 Nat Methods 20:1661; bluenote-1577/skani), FastANI 1.34+ (Jain 2018 Nat Commun 9:5114), pyani 0.3.0+ (Pritchard 2016 Anal Methods 8:12), pyskani 0.1+ (Larralde 2025), OrthoANI 1.40+ (Lee 2016 Int J Syst Evol Microbiol 66:1100), OrthoANIu 1.2+, GTDB-Tk 2.7.1+ (Chaumeil 2022 Bioinformatics 38:5315), GTDB release 220 (2024-Q3+), TYGS web (Meier-Kolthoff & Goker 2019 Nat Commun 10:2182), GGDC v3.0 (web), Mash 2.3+ (Ondov 2016 Genome Biol 17:132), Dashing 2 (Baker & Langmead 2023 Genome Res 33:1218), CompareM 0.1.2+ for AAI (Parks/Cherubini), pyANI 0.3.1+, BLAT 36+, DIAMOND 2.1+. JSpeciesWS web (Richter et al 2016 Bioinformatics 32:929).

Before using code patterns, verify installed versions match. If versions differ:

  • CLI: skani --version; fastANI --version; gtdbtk --version; mash --version; pyani --version
  • Python: pip show gtdbtk pyani

If code throws GTDB-Tk database not found, skani sketch incompatible, Mash sketch version, these tools have database-version coupling: GTDB-Tk requires the GTDB release matched to the binary version; Mash/skani sketches are forward-compatible but not always backward. Check gtdbtk check_install for database completeness.

Genome Distance and Species Delineation

"Are these genomes the same species, and what species are they?" -> Prokaryote species delineation has shifted from 16S rRNA identity (now considered insufficient at < 98.7%) to whole-genome ANI at a 95% threshold (Jain 2018 Nat Commun 9:5114; corroborating Goris 2007 and Konstantinidis 2005). The modern operational standard for taxonomy is GTDB-Tk (Chaumeil 2020/2022 Bioinformatics 38:5315), which assigns genomes to the Genome Taxonomy Database (GTDB) using ANI radius + marker-gene placement. skani (Shaw & Yu 2023 Nat Methods 20:1661) has replaced FastANI as the default ANI tool in GTDB-Tk 2.4+ for being 20-30x faster while maintaining accuracy. The 95% ANI threshold is robust but not absolute -- the species circumscription radius varies by genus (Parks 2018 Nat Biotech 36:996).

Read the full file on GitHub · 446 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 446 lines · 218 tokens per session scan A 1ae051460853

Subscribe to this mod's changes

bio-comparative-genomics-genome-distance-and-species-delineation is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 218 tokens to every session and 7,579 once invoked, about $0.0011 per session on Opus 5. A static security scan graded it A with 1 finding (makes network calls). It is 98% identical to bio-comparative-genomics-genome-distance-and-species-delineation, differing in 14 lines, and is treated as a copy.

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