bio-crispr-screens-copy-number-correction

bio-crispr-screens-copy-number-correction is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 245 tokens per session (4,668 once invoked), scanned A, a copy of bio-crispr-screens-copy-number-correction, MIT.

A correction workflow for CRISPR-Cas9 screens in cancer cell lines. Extra copies of a gene region can make it look essential because cutting the region causes DNA damage, even when the gene itself is not required.

In plain words
What is it for?
Use it to detect and correct depletion at amplified genomic regions, using CRISPRcleanR or Chronos, before downstream hit calling.
Why use it?
It reduces this copy-number-related false signal before identifying genes that truly affect cell survival or growth.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to detect and correct depletion at amplified genomic regions, using CRISPRcleanR or Chronos, before downstream hit calling.

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Install with agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-crispr-screens-copy-number-correction
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PKU-YuanGroup/OpenAI4S --skill bio-crispr-screens-copy-number-correction
Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-crispr-screens-copy-number-correction

README.md
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Your own site
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Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

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Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-crispr-screens-copy-number-correction"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-crispr-screens-copy-number-correction.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 245 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 4,668 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin 95% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00245 $0.04668
Opus 5 $0.00122 $0.02334
Sonnet 5 $0.00049 $0.00934
Haiku 4.5 $0.00024 $0.00467

Measured 9d ago against content hash 69c392164583, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

bio-crispr-screens-copy-number-correction scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

This is a copy

95% identical to bio-crispr-screens-copy-number-correction — 14 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-crispr-screens-copy-number-correction/SKILL.md · 307 lines

How it starts

The opening of the file, as written. The whole thing — 307 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: CRISPRcleanR 3.0+ (R; github.com/francescojm/CRISPRcleanR), Chronos 2.0+ (https://github.com/broadinstitute/chronos), CERES (legacy, superseded by Chronos), pandas 2.2+, numpy 1.26+, scipy 1.12+.

Before using code patterns, verify installed versions match. If versions differ:

  • R: packageVersion('CRISPRcleanR'); ?ccr.GWclean
  • Python: pip show crispr_chronos; python3 -c 'import chronos; print(chronos.__file__)'

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Copy-Number Bias Correction in CRISPR Screens

"Correct copy-number artifacts in my cancer-cell-line screen" -> Identify gene-independent depletion at amplified loci, apply CRISPRcleanR (pre-hoc, unsupervised, position-based) or Chronos (joint model, supervised with CN profile) to remove the artifact, then proceed to hit calling on corrected data.

  • R: CRISPRcleanR::ccr.GWclean() for unsupervised pre-hoc correction (no CN profile required)
  • Python: Chronos (crispr_chronos) for joint cell-population dynamics + CN modeling
  • Python: CERES (legacy, superseded by Chronos)

The Copy-Number Artifact (Mechanism)

Aguirre AJ et al 2016 Cancer Discov 6:914 and Munoz DM et al 2016 Cancer Discov 6:900 demonstrated that focal amplification regions in cancer cell lines appear systematically "essential" in CRISPR-Cas9 screens, independent of the gene's actual biology. The mechanism:

  1. A focal amplification creates 4-50+ copies of a genomic region.
  2. Each sgRNA targeting a gene in that region cuts at all copies simultaneously.
  3. Multiple cuts trigger a DNA-damage response and G2 arrest, in both TP53-mutant and TP53-wild-type lines but with larger magnitude in wild-type (Aguirre 2016).
  4. Cells arrest in G2 phase; the sgRNA appears depleted because its bearer cells don't proliferate.
  5. The depletion is proportional to the number of simultaneous cuts, not the gene's essentiality.

Read the full file on GitHub · 307 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 307 lines · 245 tokens per session scan A 69c392164583

Subscribe to this mod's changes

bio-crispr-screens-copy-number-correction is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 245 tokens to every session and 4,668 once invoked, about $0.0012 per session on Opus 5. A static security scan graded it A with 0 findings. It is 95% identical to bio-crispr-screens-copy-number-correction, differing in 14 lines, and is treated as a copy.

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