bio-sra-data

bio-sra-data is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 158 tokens per session (4,732 once invoked), scanned C, a copy of bio-sra-data, MIT.

A guide to downloading raw sequencing reads from the NCBI Sequence Read Archive and its ENA mirror. Raw reads are the original short DNA or RNA measurements produced by a sequencing machine.

In plain words
What is it for?
Fetching FASTQ files from SRA accessions, using the SRA toolkit or ENA, and handling cloud-hosted copies of sequencing data.
Why use it?
It helps you choose a suitable download source and validate the files before analysis, especially for large datasets.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one. Also seen: positional $N argument.

Needs its repository: it runs a file that does not travel with it, so clone the repository first. The line is fasterq-dump SRR12345678 --include-technical --split-files -p -O ./fastq/.

Good fit Fetching FASTQ files from SRA accessions, using the SRA toolkit or ENA, and handling cloud-hosted copies of sequencing data.

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Install

Getting it into your agent

It runs from inside its repository, so the clone comes first — what it calls does not travel with the file alone.

Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S
agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-database-access-sra-data

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-sra-data

README.md
[![agentmods](https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-sra-data/github.svg)](https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-sra-data)
Your own site
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-sra-data"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-sra-data/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-sra-data

Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-sra-data"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-sra-data.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 158 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 4,732 The whole file, excluding the scripts and references it only reads on demand.
Security scan C 2 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin 94% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00158 $0.04732
Opus 5 $0.00079 $0.02366
Sonnet 5 $0.00032 $0.00946
Haiku 4.5 $0.00016 $0.00473

Measured 9d ago against content hash 19f2d1afe6ef, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade C, and why

bio-sra-data scanned grade C with 2 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

The scan reads SKILL.md. This mod also ships 4 executable files (scripts/download_batch.sh, scripts/download_single.sh, scripts/find_sra_runs.py, …), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Recursive force deletehighDestructive command

rm -rf with a variable or a broad path is one typo away from removing the wrong tree.

# rm -rf ~/ncbi/sra/${SRR}.sra

Makes network callslowCapability

Not a fault in itself. Listed so you know the mod talks to something, and to what.

- CLI: `curl https://ftp.sra.ebi.ac.uk/...` (ENA mirror; direct FASTQ)
Origin

This is a copy

94% identical to bio-sra-data — 16 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-database-access-sra-data/SKILL.md · 386 lines

How it starts

The opening of the file, as written. The whole thing — 386 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: sra-tools 3.0+ (fasterq-dump, prefetch, vdb-validate, vdb-config), pysradb 2.2+, ENA portal API 2.0+

Before using code patterns, verify installed versions match. If versions differ:

  • CLI: fasterq-dump --version, prefetch --version
  • Python: pip show pysradb

If a flag is unrecognized or behavior changes, run <tool> --help and adapt.

SRA Data

"Download FASTQ from this SRA accession" -> Two paths exist in 2026: the SRA toolkit (NCBI's official, with prefetch + fasterq-dump) and the ENA mirror (EMBL-EBI's mirror with direct FASTQ download, often faster). For >1 TB workflows, a third path: AWS Open Data (STRIDES program) where same-region EC2 pulls SRA data with zero egress cost.

The single most impactful decision is where to pull from. SRA-direct is the default but ENA is faster more often than not, and AWS Open Data is the right answer for cloud-native analysis pipelines.

  • CLI: prefetch SRR..., fasterq-dump SRR..., vdb-validate SRR... (sra-tools)
  • CLI: curl https://ftp.sra.ebi.ac.uk/... (ENA mirror; direct FASTQ)
  • CLI: aws s3 cp s3://sra-pub-run-odp/sra/SRR.../SRR... ./SRR....sra ... (STRIDES; object is unsuffixed; same-region free)
  • Python: pysradb for metadata; subprocess for download

Required Setup

# sra-tools (toolkit)
conda install -c bioconda sra-tools           # 3.0+
fasterq-dump --version                        # confirm

# Configure cache location (default ~/ncbi/ -- often too small)
vdb-config --cfg                              # show current config
vdb-config --set /repository/user/main/public/root=/data/sra_cache

# Optional: pysradb for metadata
pip install pysradb

For STRIDES cloud:

# AWS CLI (no NCBI auth needed for public buckets)
aws s3 ls s3://sra-pub-run-odp/sra/SRR12345678/ --no-sign-request

Decision matrix: where to pull from

Source When best Speed Cost
ENA mirror (FTP/Aspera) Default for most workflows Often fastest; direct FASTQ (no SRA->FASTQ conversion needed) Free; no rate limit observed
SRA toolkit + AWS STRIDES Same-region EC2/EKS Fastest within AWS us-east-1 Free egress within region; small storage cost
SRA toolkit + GCP STRIDES Same-region GCP Compute Engine Fastest within GCP us-central1 Free egress within region
SRA-direct (prefetch + fasterq-dump) On-prem; small downloads; need SRA-format access Variable; can be slow off-peak fails Free; NCBI throttles by IP
Aspera (ascp) Institutional accounts only Faster than HTTPS on long links NCBI public Aspera retired 2019; ENA public Aspera retired ~2023; institutional use still possible

Read the full file on GitHub · 386 lines

Files

What ships with it

5 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 386 lines · 158 tokens per session scan C 19f2d1afe6ef

Subscribe to this mod's changes

bio-sra-data is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 158 tokens to every session and 4,732 once invoked, about $0.0008 per session on Opus 5. A static security scan graded it C with 2 findings (recursive force delete, makes network calls). It is 94% identical to bio-sra-data, differing in 16 lines, and is treated as a copy.

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