bio-differential-expression-de-results

bio-differential-expression-de-results is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 169 tokens per session (6,248 once invoked), scanned A, a copy of bio-differential-expression-de-results, MIT.

A guide to extracting, filtering, annotating, and exporting differential-expression results from DESeq2 or edgeR. These tools compare gene activity between groups while accounting for statistical uncertainty.

In plain words
What is it for?
Producing ranked gene lists, selecting statistically supported changes, adding gene annotations, and exporting result tables.
Why use it?
It helps interpret missing adjusted values correctly and apply suitable controls for testing thousands of genes at once.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Producing ranked gene lists, selecting statistically supported changes, adding gene annotations, and exporting result tables.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-differential-expression-de-results
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PKU-YuanGroup/OpenAI4S --skill bio-differential-expression-de-results
Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-differential-expression-de-results

README.md
[![agentmods](https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results/github.svg)](https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results)
Your own site
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-differential-expression-de-results

Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-differential-expression-de-results.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 169 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 6,248 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin 92% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00169 $0.06248
Opus 5 $0.00084 $0.03124
Sonnet 5 $0.00034 $0.01250
Haiku 4.5 $0.00017 $0.00625

Measured 9d ago against content hash 528e421042ad, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

bio-differential-expression-de-results scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

This is a copy

92% identical to bio-differential-expression-de-results — 12 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-differential-expression-de-results/SKILL.md · 384 lines

How it starts

The opening of the file, as written. The whole thing — 384 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: DESeq2 1.42+, edgeR 4.0+, IHW 1.34+, qvalue 2.34+, ashr 2.2+, AnnotationDbi 1.66+, org.Hs.eg.db 3.18+, biomaRt 2.58+, mygene 1.38+ (Python), dplyr 1.1+, openxlsx 4.2+

Before using code patterns, verify installed versions match. If versions differ:

  • R: packageVersion('<pkg>') then ?function_name to verify parameters
  • Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

DE Results

"What are my significant genes?" -> Extract DE estimates and p-values from the fitted model, handle missing padj correctly, apply FDR control appropriate to the design, and produce the table or ranked list the downstream tool actually needs.

The Single Most Important Modern Insight -- padj = NA has three distinct meanings

A NA in the padj column is not a missing value; it is a flag indicating which filter excluded the gene. The three causes -- independent filtering, Cook's distance outlier, and all-zero in a group -- have completely different remediations. Dropping all NA rows blindly silently discards real signal, most often from low-count master regulators (transcription factors expressed at ~10 counts) that pass biology but fail the data-driven baseMean threshold.

padj = NA cause DESeq2 detection What it means Fix if undesired
Independent filtering finite pvalue, NA padj, baseMean below auto threshold Removed before BH adjustment to maximize rejections at alpha results(dds, independentFiltering = FALSE) OR filterFun = ihw
Cook's distance outlier NA pvalue, NA padj, baseMean > 0, group has >=3 reps One sample has Cook's > qf(0.99, p, m-p) results(dds, cooksCutoff = FALSE)
All-zero or near-zero in a group NA pvalue AND baseMean very low Insufficient information to test Filter at preprocess time; or accept

Read the full file on GitHub · 384 lines

Files

What ships with it

4 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 384 lines · 169 tokens per session scan A 528e421042ad

Subscribe to this mod's changes

bio-differential-expression-de-results is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 169 tokens to every session and 6,248 once invoked, about $0.0008 per session on Opus 5. A static security scan graded it A with 0 findings. It is 92% identical to bio-differential-expression-de-results, differing in 12 lines, and is treated as a copy.

Related

Other skills, from other repositories

boltz-structure-prediction

Boltz-1 / Boltz-2 structure prediction for proteins, complexes, and ligand-aware validation. Use this skill when: (1) Predicting protein complex structures, (2) Validating designed binders, (3) Need open-source alternative to AF2, (4) Predicting protein-ligand complexes, (5) Using local GPU resources. For QC…

zongtingwei/Bioclaw_Skills_Hub · 121 tokens

imaging-data-commons

Query and download public cancer imaging data from NCI Imaging Data Commons using idc-index. Use for accessing large-scale radiology (CT, MR, PET) and pathology datasets for AI training or research. No authentication required. Query by metadata, visualize in browser, check licenses.

synthetic-sciences/openscience · 62 tokens

flow-cytometry-analysis

Complete flow cytometry analysis pipeline. FCS file handling, compensation, manual/automated gating, immunophenotyping, CFSE proliferation analysis, cell cycle analysis (Dean-Jett-Fox), and apoptosis assays. Extends flowio with analytical workflows. For raw FCS parsing only use flowio.

synthetic-sciences/openscience · 67 tokens

scientific-critical-thinking

Evaluate scientific claims and evidence quality. Use for assessing experimental design validity, identifying biases and confounders, applying evidence grading frameworks (GRADE, Cochrane Risk of Bias), or teaching critical analysis. Best for understanding evidence quality, identifying flaws. For formal peer review…

xintaofei/codeg · 63 tokens

cellxgene-census

Query the CELLxGENE Census (61M+ cells) programmatically. Use when you need expression data across tissues, diseases, or cell types from the largest curated single-cell atlas. Best for population-scale queries, reference atlas comparisons. For analyzing your own data use scanpy or scvi-tools.

synthetic-sciences/openscience · 67 tokens

glycobiology

Glycosylation site prediction and glycobiology analysis. N-glycosylation motif finding, O-glycosylation hotspot prediction, glycan structure resources. Lightweight, pure Python. For protein function queries use uniprot-database; for structure analysis use alphafold-database.

synthetic-sciences/openscience · 67 tokens