bulkrna-read-qc

bulkrna-read-qc is a skill for Claude Code, Codex from TianGzlab/OmicsClaw. It costs 71 tokens per session (819 once invoked), scanned A, original, Apache-2.0.

A quality check for raw FASTQ files, the standard text files containing DNA or RNA sequencing reads and their quality scores, before alignment.

In plain words
What is it for?
Use it on single- or paired-end bulk RNA-seq FASTQ or compressed FASTQ files to produce quality tables, charts, and a report.
Why use it?
It helps decide whether the reads need trimming by showing base quality, GC content, adapter signals, read lengths, and Q20/Q30 quality fractions.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it on single- or paired-end bulk RNA-seq FASTQ or compressed FASTQ files to produce quality tables, charts, and a report.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/tiangzlab/omicsclaw/bulkrna-read-qc
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add TianGzlab/OmicsClaw --skill bulkrna-read-qc
Clone the repo
git clone --depth 1 https://github.com/TianGzlab/OmicsClaw

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bulkrna-read-qc

README.md
[![agentmods](https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/bulkrna-read-qc/github.svg)](https://agentmods.dev/skills/tiangzlab/omicsclaw/bulkrna-read-qc)
Your own site
<a href="https://agentmods.dev/skills/tiangzlab/omicsclaw/bulkrna-read-qc"><img src="https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/bulkrna-read-qc/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bulkrna-read-qc

Your own site · 80×15
<a href="https://agentmods.dev/skills/tiangzlab/omicsclaw/bulkrna-read-qc"><img src="https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/bulkrna-read-qc.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 71 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 819 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector warn 7 Sept 2026
SkillSpector: 1 finding, up to high

These are SkillSpector’s own severities. On a checked sample its high-severity flags on skills were ~96% false positives — a documented command, a public API, a “never do X” rule — so we show them as a caution to read, not a verdict. Why →

  • high Rogue Agent · line 3
    Skill modifies its own code, configuration, or behavior at runtime. Self-modification enables an agent to escalate privileges, disable safety constraints, or install persistent backdoors.
    Fix: Prevent the skill from modifying its own code, SKILL.md, or configuration files. Treat skill files as read-only at runtime.
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00071 $0.00819
Opus 5 $0.00036 $0.00409
Sonnet 5 $0.00014 $0.00164
Haiku 4.5 $0.00007 $0.00082

Measured 10d ago against content hash 471128b6ec46, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-10, from the pricing page.

Security

Grade A, and why

bulkrna-read-qc scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 10d ago.

The scan reads SKILL.md. This mod also ships 1 executable file (bulkrna_read_qc.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

skills/bulkrna/bulkrna-read-qc/SKILL.md · 79 lines

How it starts

The opening of the file, as written. The whole thing — 79 lines — stays where its author put it; the contents beside it link to each section on GitHub.

bulkrna-read-qc

When to use

Run as the first step on raw bulk RNA-seq FASTQ files (single or paired-end) before aligning. Reports per-base Phred quality, GC content, adapter contamination signals, read length distribution, and Q20/Q30 fractions — the metrics needed to decide whether trimming is worth the trouble.

Inputs & Outputs

Inputs

  • File types: .fastq, .fq

Outputs

  • tables/qc_summary.csv
  • figures/gc_content.png
  • figures/per_base_quality.png
  • figures/quality_score_distribution.png
  • figures/read_length_distribution.png
  • report.md
  • result.json

Flow

  1. Open the FASTQ (auto-decompresses .gz per bulkrna_read_qc.py:70).
  2. Sample reads, decode Phred quality from header line 4 of each record.
  3. Compute per-base quality, GC content, length distribution, adapter motif counts.
  4. Render figures and write report.md + result.json.

Gotchas

  • This is a pure-Python reimplementation of FastQC core metrics, not FastQC itself. Coverage of the more obscure FastQC modules (overrepresented sequences, k-mer enrichment, per-tile quality) is intentionally omitted to keep the skill dependency-free. For full FastQC parity, run FastQC directly and feed the report into MultiQC.
  • Phred encoding is assumed to be Phred+33 (Sanger / Illumina 1.8+). Older Illumina 1.3–1.7 platforms used Phred+64 — the per-base quality values will look ~31 points too high if such input is fed in unchanged. Confirm the source platform before trusting Q20/Q30 numbers.
  • .gz detection is filename-suffix only (bulkrna_read_qc.py:70 checks .endswith(".gz")). A gzipped file misnamed without .gz will be opened as text and silently produce garbage; rename or symlink before running.

Key CLI

python omicsclaw.py run bulkrna-read-qc --demo
python omicsclaw.py run bulkrna-read-qc --input reads.fastq.gz --output results/

Read the full file on GitHub · 79 lines

Files

What ships with it

5 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 10d ago First seen · 79 lines · 71 tokens per session scan A 471128b6ec46

Subscribe to this mod's changes

bulkrna-read-qc is a skill published in the GitHub repository TianGzlab/OmicsClaw (160 stars, last pushed 1mo ago), licensed Apache-2.0. It adds 71 tokens to every session and 819 once invoked, about $0.0004 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

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