genome-alignment-and-contact-matrix-construction

genome-alignment-and-contact-matrix-construction is a skill for Claude Code, Codex from HolobiomicsLab/asb-skill-collections. It costs 75 tokens per session (1,660 once invoked), scanned A, original, Apache-2.0.

A pipeline that aligns raw paired-end Hi-C reads, removes duplicate data, normalizes contacts, and creates a .hic contact map. Hi-C is a sequencing method for measuring which genome regions are near one another in three-dimensional space.

In plain words
What is it for?
Use it as the starting point for a supported paired-end Hi-C experiment when no processed contact map exists.
Why use it?
Raw FASTQ files cannot be used directly for contact-map visualization or loop analysis. This processing turns them into a normalized map for those tasks.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it as the starting point for a supported paired-end Hi-C experiment when no processed contact map exists.

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Install with agentmods
npx agentmods add skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add HolobiomicsLab/asb-skill-collections --skill genome-alignment-and-contact-matrix-construction
Clone the repo
git clone --depth 1 https://github.com/HolobiomicsLab/asb-skill-collections

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for genome-alignment-and-contact-matrix-construction

README.md
[![agentmods](https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction/github.svg)](https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction)
Your own site
<a href="https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction"><img src="https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for genome-alignment-and-contact-matrix-construction

Your own site · 80×15
<a href="https://agentmods.dev/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction"><img src="https://agentmods.dev/badge/skills/holobiomicslab/asb-skill-collections/genome-alignment-and-contact-matrix-construction.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 75 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,660 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector warn 7 Sept 2026
SkillSpector: 1 finding, up to medium

These are SkillSpector’s own severities. On a checked sample its high-severity flags on skills were ~96% false positives — a documented command, a public API, a “never do X” rule — so we show them as a caution to read, not a verdict. Why →

  • medium Output Handling · line 88
    Output size or generation rate is not bounded. Unbounded output enables denial-of-service through resource exhaustion, log flooding, or context-window stuffing.
    Fix: Set explicit limits on output length, generation count, and rate. Use max_tokens and truncation to prevent unbounded output.
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00075 $0.01660
Opus 5 $0.00037 $0.00830
Sonnet 5 $0.00015 $0.00332
Haiku 4.5 $0.00007 $0.00166

Measured 9d ago against content hash 6d12dbe446b8, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

genome-alignment-and-contact-matrix-construction scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

collections/epigenomics/v1/skills/genome-alignment-and-contact-matrix-construction/SKILL.md · 108 lines

How it starts

The opening of the file, as written. The whole thing — 108 lines — stays where its author put it; the contents beside it link to each section on GitHub.

genome-alignment-and-contact-matrix-construction

Summary

Process raw Hi-C FASTQ sequencing data through read alignment, deduplication, and normalization to generate kilobase-resolution contact matrices in .hic format. This skill is essential when starting from FASTQ reads and needing to construct quantitative genome-wide interaction maps for downstream analysis.

When to use

You have raw Hi-C FASTQ files from a kilobase-resolution Hi-C experiment and need to produce a processed Hi-C contact map (.hic file) for visualization, loop calling, or chromatin structure analysis. This is the entry point for any Hi-C dataset that has not yet been aligned and normalized.

When NOT to use

  • Input Hi-C data is already in .hic format or pre-processed contact matrix form — use downstream feature annotation tools instead.
  • You have single-end Hi-C reads or non-standard pairing; Juicer is optimized for paired-end sequencing.
  • Your restriction enzyme or genome is not supported without custom configuration of the pipeline.

Inputs

  • Raw Hi-C FASTQ files (paired-end sequencing reads)
  • Reference genome FASTA file
  • Chromosome sizes file (chrom.sizes)
  • Restriction enzyme recognition site file

Outputs

  • .hic file (processed Hi-C contact map with normalized interactions)
  • Intermediate alignment files (in aligned/ directory)
  • Pipeline statistics and logs

How to apply

Clone the Juicer repository (either stable Juicer 1.6 or development Juicer 2 depending on your requirements) and configure the pipeline with your reference genome, restriction enzyme recognition site, and computational resources (threads and memory allocation). Organize raw FASTQ files in a designated fastq/ subdirectory and run juicer.sh with appropriate flags (e.g., -g for genome ID, -y for restriction site file, -z for reference genome). The pipeline automatically handles read alignment via BWA, contact matrix construction, and normalization, producing a .hic output file. Verify success by confirming the .hic file is generated without errors and contains valid Hi-C contact data.

Read the full file on GitHub · 108 lines

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 108 lines · 75 tokens per session scan A 6d12dbe446b8

Subscribe to this mod's changes

genome-alignment-and-contact-matrix-construction is a skill published in the GitHub repository HolobiomicsLab/asb-skill-collections (15 stars, last pushed yesterday), licensed Apache-2.0. It adds 75 tokens to every session and 1,660 once invoked, about $0.0004 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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