bio-blast-searches

bio-blast-searches is a skill for Claude Code, Codex from PKU-YuanGroup/OpenAI4S. It costs 130 tokens per session (4,334 once invoked), scanned A, a copy of bio-blast-searches, MIT.

A Python-based tool for sending DNA or protein sequences to NCBI BLAST, which compares them with database sequences to find similar ones. It covers choosing the right search type and reading statistical scores such as E-values.

In plain words
What is it for?
Use it to identify unknown sequences, find related sequences, and compare a small number of DNA or protein sequences with NCBI databases.
Why use it?
It removes the guesswork around selecting a BLAST search and interpreting its results. It also explains how to avoid misleading hit limits and when to use a local search for larger batches.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to identify unknown sequences, find related sequences, and compare a small number of DNA or protein sequences with NCBI databases.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/pku-yuangroup/openai4s/bio-database-access-blast-searches
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add PKU-YuanGroup/OpenAI4S --skill bio-database-access-blast-searches
Clone the repo
git clone --depth 1 https://github.com/PKU-YuanGroup/OpenAI4S

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for bio-blast-searches

README.md
[![agentmods](https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches/github.svg)](https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches)
Your own site
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for bio-blast-searches

Your own site · 80×15
<a href="https://agentmods.dev/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches"><img src="https://agentmods.dev/badge/skills/pku-yuangroup/openai4s/bio-database-access-blast-searches.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 130 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 4,334 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe.
Origin 98% copy Near-identical to another mod in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00130 $0.04334
Opus 5 $0.00065 $0.02167
Sonnet 5 $0.00026 $0.00867
Haiku 4.5 $0.00013 $0.00433

Measured 9d ago against content hash 87f74ebe1aa1, method: parsed. Prices are Anthropic first-party input rates as of 2026-09-12, from the pricing page.

Security

Grade A, and why

bio-blast-searches scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 9d ago.

The scan reads SKILL.md. This mod also ships 3 executable files (scripts/basic_blast.py, scripts/blastp_filtered.py, scripts/save_and_parse.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

Origin

This is a copy

98% identical to bio-blast-searches — 12 lines differ, which has more behind it and is treated as the original. This page carries a canonical link to it rather than competing with it.

skills/bioskills/bio-database-access-blast-searches/SKILL.md · 319 lines

How it starts

The opening of the file, as written. The whole thing — 319 lines — stays where its author put it; the contents beside it link to each section on GitHub.

Version Compatibility

Reference examples tested with: BioPython 1.83+, NCBI BLAST+ 2.15+

Before using code patterns, verify installed versions match. If versions differ:

  • Python: pip show biopython then help(Bio.Blast.NCBIWWW.qblast) to check signatures
  • CLI: blastn -version then blastn -help

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

BLAST Searches (Remote)

"Find similar sequences in NCBI's database" -> Submit a query to NCBI's remote BLAST servers; receive a Request ID (RID); poll for completion; parse the XML hit table. Best for one-off identification of a few sequences. For >50 sequences, switch to local-blast or DIAMOND/MMseqs2 in remote-homology.

The two most consequential decisions: which program (defines query+target molecule types and word-size defaults) and which database (defines the search space and therefore E-value baselines). The third most important: do NOT misuse max_target_seqs -- it is an early-termination heuristic, not a "give me the top N hits" filter (Shah et al. 2019).

  • Python: NCBIWWW.qblast(program, db, sequence) + NCBIXML.read(handle) (BioPython)
  • CLI: blastn -remote -db nt -query seq.fa -out hits.xml -outfmt 5 (BLAST+)
  • Web: https://blast.ncbi.nlm.nih.gov/Blast.cgi (RID lookup)

Required Setup

from Bio.Blast import NCBIWWW, NCBIXML
from Bio import SeqIO

No API key needed for remote BLAST itself, but NCBI's general rate-limit ethic still applies -- one search at a time, polite waiting, no parallelism.

Program decision (query vs database molecule)

Program Query Target Word size default Use case
blastn DNA DNA 11 General DNA similarity
megablast DNA DNA 28 High-identity DNA (>=95%) -- PCR primer hits, contamination
dc-megablast DNA DNA 11 (discontiguous) Cross-species mRNA (sensitive, gapped)
blastp Protein Protein 3 (6 also valid) General protein homology
blastx DNA Protein 3 Translated DNA query vs protein DB; ORF discovery
tblastn Protein DNA 3 Protein query vs translated DB; find unannotated CDS
tblastx DNA DNA 3 (both translated) Most expensive; deep cross-species coding similarity
psiblast Protein Protein 3 Iterative PSSM-based remote homology -- see remote-homology

Read the full file on GitHub · 319 lines

Files

What ships with it

4 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 9d ago First seen · 319 lines · 130 tokens per session scan A 87f74ebe1aa1

Subscribe to this mod's changes

bio-blast-searches is a skill published in the GitHub repository PKU-YuanGroup/OpenAI4S (407 stars, last pushed yesterday), licensed MIT. It adds 130 tokens to every session and 4,334 once invoked, about $0.0006 per session on Opus 5. A static security scan graded it A with 0 findings. It is 98% identical to bio-blast-searches, differing in 12 lines, and is treated as a copy.

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