spatial-condition-comparison

spatial-condition-comparison is a skill for Claude Code, Codex from ShangBioLab/SpatialClaw. It costs 22 tokens per session (1,890 once invoked), scanned A, original, Apache-2.0.

A tool for comparing experimental conditions, such as treatment and control, in spatial transcriptomics data using pseudobulk statistics. Pseudobulk combines counts from cells in each sample and region so samples—not individual cells—are treated as the biological replicates.

In plain words
What is it for?
Use it to aggregate raw counts by sample and region, test condition differences with PyDESeq2-style analysis, correct for multiple testing, and produce differential-expression tables and plots.
Why use it?
It avoids overstating significance by treating many cells from one sample as independent observations, and accounts for differences between samples.

Skill for Claude CodeCodex

Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

agentmods
npx agentmods add skills/shangbiolab/spatialclaw/spatial-condition-comparison
Any agent
npx skills add ShangBioLab/SpatialClaw --skill spatial-condition-comparison
Clone the repo
git clone --depth 1 https://github.com/ShangBioLab/SpatialClaw

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for spatial-condition-comparison

README.md
[![agentmods](https://agentmods.dev/badge/skills/shangbiolab/spatialclaw/spatial-condition-comparison.svg)](https://agentmods.dev/skills/shangbiolab/spatialclaw/spatial-condition-comparison)
Your own site
<a href="https://agentmods.dev/skills/shangbiolab/spatialclaw/spatial-condition-comparison"><img src="https://agentmods.dev/badge/skills/shangbiolab/spatialclaw/spatial-condition-comparison.svg" alt="Measured on agentmods" height="20"></a>
Per session 22 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,890 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. Scan, not verified.
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5 $0.00022 $0.01890
Opus 5 $0.00011 $0.00945
Sonnet 5 $0.00004 $0.00378
Haiku 4.5 $0.00002 $0.00189

Measured 4d ago against content hash 2210b76a482d, method: parsed. Prices are Anthropic first-party input rates as of 2026-08-30, from the pricing page.

Security

Grade A, and why

spatial-condition-comparison scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 4d ago.

The scan reads SKILL.md. This mod also ships 2 executable files (spatial_condition_comparison.py, tests/test_spatial_condition_comparison.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

skills/spatial/spatial-condition-comparison/SKILL.md · 184 lines

How it starts

The opening of the file, as written. The whole thing — 184 lines — stays where its author put it; the contents beside it link to each section on GitHub.

⚖️ Spatial Condition

You are Spatial Condition, a specialised SPATIALCLAW agent for comparing experimental conditions in spatial transcriptomics data. Your role is to perform proper multi-sample pseudobulk differential expression analysis between treatment groups.

Why This Exists

  • Without it: Users run per-cell Wilcoxon tests between conditions, inflating significance due to pseudoreplication
  • With it: Proper pseudobulk aggregation + DESeq2-style statistics that respect sample-level variability
  • Why SPATIALCLAW: Handles the full pseudobulk pipeline automatically with spatial context awareness

Workflow

  1. Calculate: Aggregate pseudobulk representations of annotated regions.
  2. Execute: Run condition-specific statistical tests (e.g., Deseq2, EdgeR logic).
  3. Assess: Perform multiple hypothesis correction to minimize false discovery.
  4. Generate: Output DE tables specific to condition differentials.
  5. Report: Synthesize report with volcano and condition plots.

Core Capabilities

  1. Pseudobulk aggregation: Sum raw counts per sample x cluster to create proper biological replicates. Uses adata.layers["counts"] (raw)
  2. PyDESeq2 testing (default): Negative-binomial GLM on raw integer pseudobulk counts (preferred for >= 3 samples/condition)
  3. Wilcoxon fallback: Non-parametric rank-sum on internally computed log-CPM from pseudobulk counts (for 2-3 samples/condition, or as explicit --method wilcoxon)
  4. Automatic fallback: If PyDESeq2 fails for a cluster, automatically falls back to Wilcoxon
  5. Per-cluster analysis: Run condition comparison within each cluster to find cluster-specific responses

Input Formats

Format Extension Required Fields Example
AnnData (preprocessed) .h5ad X (normalised), layers["counts"] (raw), obs[condition_key], obs[sample_key] multi_sample.h5ad

Input Matrix Convention

This skill has a multi-step pipeline where different steps use different input matrices:

Read the full file on GitHub · 184 lines

Files

What ships with it

2 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 4d ago First seen · 184 lines · 22 tokens per session scan A 2210b76a482d

Subscribe to this mod's changes

spatial-condition-comparison is a skill published in the GitHub repository ShangBioLab/SpatialClaw (11 stars, last pushed 3mo ago), licensed Apache-2.0. It adds 22 tokens to every session and 1,890 once invoked, about $0.0001 per session on Opus 5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-08-30.

Related

Other skills, from other repositories

spatial-condition

Load when comparing two or more experimental conditions (treatment vs control) on a multi-sample preprocessed spatial AnnData via PyDESeq2 pseudobulk or Wilcoxon DE — needs obs[conditionkey], obs[samplekey], and cluster labels. Skip when running per-cluster DE on one condition (use spatial-de); comparing two slices…

TianGzlab/OmicsClaw · 83 tokens

pseudobulk_design_helper

Recommend a replicate-aware pseudobulk strategy for single-cell differential expression and explain the design choices.

Azealoo/miniAgent · 26 tokens

spatial-de

Load when ranking spatial cluster markers or comparing two spatial groups in spatial transcriptomics. Skip when the data is single-cell (use sc-de); bulk (use bulkrna-de); spatially variable expression discovery (use spatial-genes).

TianGzlab/OmicsClaw · 51 tokens

bulkrna-de

Load when comparing gene expression between two conditions in bulk RNA-seq count data. Skip when the data is single-cell (use sc-de); spatial (use spatial-de); you need exon-level alternative splicing (use bulkrna-splicing).

TianGzlab/OmicsClaw · 54 tokens

rna-seq-analysis

Bulk RNA-seq analysis pipeline covering alignment (STAR), quantification (Salmon, featureCounts), and differential expression (DESeq2). Triggers on RNA-seq, STAR, Salmon, DESeq2, differential expression, gene expression, tximport, featureCounts, transcriptomics, "bulk RNA-seq", "STAR alignment", "Salmon…

awslabs/hcls-agent-skills · 103 tokens

differential_expression_helper

Interpret a differential expression result with replicate-aware context, likely confounders, and the next analysis decision.

Azealoo/miniAgent · 27 tokens