FBA simulation agent. Runs standard FBA, parsimonious FBA, Flux Variability Analysis, and gene/reaction knockout simulations using COBRApy. Use after a validated metabolic model is available and the user wants to compute growth rates, flux distributions, or knockout phenotypes.
Metabolic flux analysis agent. Performs gene essentiality analysis, phenotypic phase plane construction, flux sampling, and subsystem-level pathway analysis. Use after FBA simulations are complete and the user wants deeper phenotypic characterisation or to identify metabolic engineering targets.
Metabolic phenotype interpretation and publication figure agent. Compares wild-type vs. mutant flux distributions, predicts maximum theoretical product yields, identifies metabolic bottlenecks, and generates publication-quality metabolic maps and charts. Use after flux analysis is complete and the user needs…