sc-fastq-qc

sc-fastq-qc is a skill for Claude Code, Codex from TianGzlab/OmicsClaw. It costs 53 tokens per session (1,100 once invoked), scanned A, original, Apache-2.0.

A quality check for raw single-cell RNA sequencing FASTQ files, which contain the sequencing reads before they are converted into gene counts. It summarises read quality, GC content, adapters, and read length.

In plain words
What is it for?
Use it to inspect individual files or sample collections and generate per-base, per-file, and overall quality reports.
Why use it?
It can reveal poor reads or technical problems before the data is counted and analysed.

Skill for Claude CodeCodex

Written for no agent in particular: nothing here depends on one.

Good fit Use it to inspect individual files or sample collections and generate per-base, per-file, and overall quality reports.

Compare 6 skills from other repositories ↓
Install with agentmods
npx agentmods add skills/tiangzlab/omicsclaw/sc-fastq-qc
Install

Getting it into your agent

One page per mod, every tool's command on it. A separate URL per tool would split the same page into five that compete with each other.

Any agent
npx skills add TianGzlab/OmicsClaw --skill sc-fastq-qc
Clone the repo
git clone --depth 1 https://github.com/TianGzlab/OmicsClaw

Made for: Claude Code, Codex.

Wrote this? Show the measurements

A badge with what this costs and how it scanned, read live from this page, so it follows the numbers instead of freezing them. Markdown for a README, HTML for a documentation site or a project page.

agentmods badge for sc-fastq-qc

README.md
[![agentmods](https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/sc-fastq-qc/github.svg)](https://agentmods.dev/skills/tiangzlab/omicsclaw/sc-fastq-qc)
Your own site
<a href="https://agentmods.dev/skills/tiangzlab/omicsclaw/sc-fastq-qc"><img src="https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/sc-fastq-qc/github.svg" alt="Measured on agentmods" height="20"></a>

Or the 80×15 button, for a site that already has a row of RSS and ATOM ones. Only the verdict fits; the numbers stay here.

agentmods 80×15 button for sc-fastq-qc

Your own site · 80×15
<a href="https://agentmods.dev/skills/tiangzlab/omicsclaw/sc-fastq-qc"><img src="https://agentmods.dev/badge/skills/tiangzlab/omicsclaw/sc-fastq-qc.svg" alt="Reviewed on agentmods" width="80" height="20"></a>
Per session 53 Skills are progressive disclosure: only the name and description are preloaded; the body loads when the skill is used.
When invoked 1,100 The whole file, excluding the scripts and references it only reads on demand.
Security scan A 0 findings. A grade says what 26 rules found in the file — not that it is safe. Third-party audits
  • NVIDIA SkillSpector warn 7 Sept 2026
SkillSpector: 1 finding, up to high

These are SkillSpector’s own severities. On a checked sample its high-severity flags on skills were ~96% false positives — a documented command, a public API, a “never do X” rule — so we show them as a caution to read, not a verdict. Why →

  • high Rogue Agent · line 3
    Skill modifies its own code, configuration, or behavior at runtime. Self-modification enables an agent to escalate privileges, disable safety constraints, or install persistent backdoors.
    Fix: Prevent the skill from modifying its own code, SKILL.md, or configuration files. Treat skill files as read-only at runtime.
How audits are shown
Origin original No closer match found in the catalogue.
Token cost

What it costs to keep this loaded

Counted locally with the o200k_base tokenizer, which is exact for GPT models; Claude uses its own tokenizer and its counts differ. Treat this as one consistent yardstick across the catalogue rather than a bill. Prices are per million input tokens.

ModelPer sessionOnce invoked
Fable 5.1 $0.00053 $0.01100
Opus 5.5 $0.00021 $0.00440
Sonnet 5.5 $0.00011 $0.00220
Haiku 4.5 $0.00005 $0.00110

Measured 17d ago against content hash b869c908948a, method: parsed. Prices are Anthropic first-party input rates as of 2026-10-07, from the pricing page.

Security

Grade A, and why

sc-fastq-qc scanned grade A with 0 findings against 26 rules in 11 categories — prompt injection, anti-refusal, data exfiltration, privilege escalation, supply chain, agent snooping, system-prompt leakage, SSRF and excessive agency — measured 17d ago.

The scan reads SKILL.md. This mod also ships 3 executable files (sc_fastq_qc.py, tests/test_fastqc_paths.py, tests/test_sc_fastq_qc.py), listed below but not scanned — reading those needs a real analyzer, not pattern matching.

A static scan of the body, not an audit. Every finding is printed with the line that produced it so you can judge whether it matters here. A mod is markdown that instructs an agent; that is exactly why what it instructs is worth reading.

Nothing flagged

None of the 26 patterns this scan looks for appear in this file: no shell pipes, no recursive deletes, no credential paths, no hidden text, no instruction-override or anti-refusal phrasing, no agent-config snooping. That is not a guarantee, it is the absence of the things that are checkable.

skills/singlecell/scrna/sc-fastq-qc/SKILL.md · 106 lines

How it starts

The opening of the file, as written. The whole thing — 106 lines — stays where its author put it; the contents beside it link to each section on GitHub.

sc-fastq-qc

Use from a step

This is CLI_ONLY: it reads FASTQ files and optionally starts FastQC/MultiQC.

from skills._sdk.notebook import run_cli

run_cli("sc-fastq-qc", "--input", "data/sample_R1.fastq.gz",
        "--read2", "data/sample_R2.fastq.gz",
        inputs=["data/sample_R1.fastq.gz", "data/sample_R2.fastq.gz"])

The demo renders synthetic summary tables; it does not read FASTQ or test the external tools. There is no _api.py for this skill.

When to use

The user has raw scRNA-seq FASTQ files (one or more, or a directory of samples) and wants per-file / per-sample / per-base quality summaries before running sc-count or cellranger. Python summaries always run. FastQC and MultiQC add reports when installed; a tool that is present but fails is a hard error, not a fallback to a successful Python-only run.

Inputs & Outputs

Inputs

  • Input kinds: file, directory
  • Modalities: scrna
  • File types: .fastq, .fq, and their .gz variants
  • FASTQ structure: valid first record
  • Directory layouts (any): fastq-collection

Outputs

  • tables/fastq_per_base_quality.csv
  • tables/fastq_per_file_summary.csv
  • tables/fastq_per_sample_summary.csv
  • figures/fastq_file_quality.png
  • figures/fastq_q30_summary.png
  • figures/fastq_read_structure.png
  • figures/per_base_quality.png
  • figures/manifest.json, figure_data/manifest.json, plot-data CSV files
  • reproducibility/commands.sh, reproducibility/requirements.txt
  • Optional external reports under artifacts/fastqc/ and artifacts/multiqc/
  • report.md
  • result.json

Flow

  1. Discover FASTQ files and choose one sample (--sample disambiguates a directory).
  2. Summarize the first --max-reads records per file in Python.
  3. Run FastQC when present, then MultiQC when both tools are present.
  4. Render four figures from the Python summaries; external HTML is separate.
  5. Write three tables, report.md and result.json.

Gotchas

  • --max-reads 20000 caps Python summaries only. tables/fastq_per_file_summary.csv records sampled depth. FastQC processes the full files. This is a prefix sample, not random sampling.
  • --r-enhanced is accepted but produces no R plots. This skill emits Python figures only. Pass freely, expect no R Enhanced output.
  • figures/manifest.json records per-figure status; result.json lists external tool availability and commands under data.external_tools.
  • _lib/upstream.py:choose_fastq_sample rejects ambiguous multi-sample directories. Run once per sample with --sample; one invocation does not batch every sample.

Read the full file on GitHub · 106 lines

Files

What ships with it

7 files beside SKILL.md in the same directory: the scripts, references and assets a skill reads on demand. Not counted in the per-session cost; read them before you install if any of them is executable.

Changes

What this file has done since we first saw it

Hashed on every crawl. A supply-chain change to an agent config is a question of when, not whether, so the history is kept rather than the latest state alone.

  1. 17d ago Changed · -2 lines b869c908948a
  2. 1mo ago First seen · 108 lines · 53 tokens per session scan A 88a59c95dab8

Subscribe to this mod's changes

sc-fastq-qc is a skill published in the GitHub repository TianGzlab/OmicsClaw (161 stars, last pushed 17d ago), licensed Apache-2.0. It adds 53 tokens to every session and 1,100 once invoked, about $0.0002 per session on Opus 5.5. A static security scan graded it A with 0 findings. No closer match exists in the catalogue, so it is treated as the original; first seen 2026-09-03.

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